BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15831

Title: chemical shift assignments and heteronuclear NOE of the N-terminal domain of the human Y2-receptor   PubMed: 19125388

Authors: Zerbe, Oliver; Walser, Reto; Zou, Chao; Kumaran, Sowmini

Citation: Zou, Chao; Kumaran, Sowmini; Walser, Reto; Zerbe, Oliver. "Properties of the N-terminal domains from Y receptors probed by NMR spectroscopy."  J. Pept. Sci. 15, 184-191 (2009).

Assembly members:
N-Y2, polymer, 50 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
N-Y2: MGPIGAEADENQTVEEMKVE QYGPQTTPRGELVPDPEPEL IDSTKLIEVQ

Data sets:
Data typeCount
15N chemical shifts43
1H chemical shifts43
heteronuclear NOE values43

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-Y21

Entities:

Entity 1, N-Y2 50 residues - Formula weight is not available

1   METGLYPROILEGLYALAGLUALAASPGLU
2   ASNGLNTHRVALGLUGLUMETLYSVALGLU
3   GLNTYRGLYPROGLNTHRTHRPROARGGLY
4   GLULEUVALPROASPPROGLUPROGLULEU
5   ILEASPSERTHRLYSLEUILEGLUVALGLN

Samples:

sample_1: N-Y2, [U-15N], 0.2 mM; MES 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_2: N-Y2, [U-15N], 0.4 mM; MES 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N NOEsample_2isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

DBJ BAG74149
GB AAA93170 AAB04120 AAB07760 AAC50281 AAC51115
REF NP_000901 NP_001012655 NP_001028004 XP_002832879 XP_003257918
SP P49146 Q5IS62 Q9GK74