BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15803

Title: RalB in complex with its effector RLIP76   PubMed: 19636899

Authors: Mott, Helen

Citation: Fenwick, R. Bryn; Prasannan, Sunil; Campbell, Louise; Evetts, Katrina; Nietlispach, Daniel; Owen, Darerca; Mott, Helen. "1H, 13C and 15N resonance assignments for the active conformation of the small G proteins RalB in complex with its effector RLIP76"  Biomol. NMR Assignments 2, 179-182 (2008).

Assembly members:
RalB, polymer, 188 residues, Formula weight is not available
RLIP76, polymer, 56 residues, Formula weight is not available
GNP, non-polymer, 522.196 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RalB: GSHMAANKSKGQSSLALHKV IMVGSGGVGKSALTLQFMYD EFVEDYEPTKADSYRKKVVL DGEEVQIDILDTAGLEDYAA IRDNYFRSGEGFLLVFSITE HESFTATAEFREQILRVKAE EDKIPLLVVGNKSDLEERRQ VPVEEARSKAEEWGVQYVET SAKTRANVDKVFFDLMREIR TKKMSENK
RLIP76: GSETQAGIKEEIRRQEFLLN SLHRDLQGGIKDLSKEERLW EVQRILTALKRKLREA

Data sets:
Data typeCount
13C chemical shifts759
15N chemical shifts193
1H chemical shifts1283

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RalB1
2RLIP762
3GNP3

Entities:

Entity 1, RalB 188 residues - Formula weight is not available

1   GLYSERHISMETALAALAASNLYSSERLYS
2   GLYGLNSERSERLEUALALEUHISLYSVAL
3   ILEMETVALGLYSERGLYGLYVALGLYLYS
4   SERALALEUTHRLEUGLNPHEMETTYRASP
5   GLUPHEVALGLUASPTYRGLUPROTHRLYS
6   ALAASPSERTYRARGLYSLYSVALVALLEU
7   ASPGLYGLUGLUVALGLNILEASPILELEU
8   ASPTHRALAGLYLEUGLUASPTYRALAALA
9   ILEARGASPASNTYRPHEARGSERGLYGLU
10   GLYPHELEULEUVALPHESERILETHRGLU
11   HISGLUSERPHETHRALATHRALAGLUPHE
12   ARGGLUGLNILELEUARGVALLYSALAGLU
13   GLUASPLYSILEPROLEULEUVALVALGLY
14   ASNLYSSERASPLEUGLUGLUARGARGGLN
15   VALPROVALGLUGLUALAARGSERLYSALA
16   GLUGLUTRPGLYVALGLNTYRVALGLUTHR
17   SERALALYSTHRARGALAASNVALASPLYS
18   VALPHEPHEASPLEUMETARGGLUILEARG
19   THRLYSLYSMETSERGLUASNLYS

Entity 2, RLIP76 56 residues - Formula weight is not available

The first two residues are a tag remnant. The protein starts at E393 and ends at A446

1   GLYSERGLUTHRGLNALAGLYILELYSGLU
2   GLUILEARGARGGLNGLUPHELEULEUASN
3   SERLEUHISARGASPLEUGLNGLYGLYILE
4   LYSASPLEUSERLYSGLUGLUARGLEUTRP
5   GLUVALGLNARGILELEUTHRALALEULYS
6   ARGLYSLEUARGGLUALA

Entity 3, GNP - C10 H17 N6 O13 P3 - 522.196 Da.

1   GNP

Samples:

sample_1: RalB, [U-99% 15N], 0.8 mM; RLIP76 0.8 mM; H2O 90%; D2O 10%

sample_2: RalB, [U-99% 13C; U-99% 15N], 0.8 mM; RLIP76 0.8 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.16 M; pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1

Software:

AZARA, Boucher - processing

Analysis, Wranken etal - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 15230 15524 15525
PDB
DBJ BAE02438 BAG35360 BAG64302 BAJ20798
EMBL CAA33119 CAH93398
GB AAA60250 AAH18163 AAI46174 AAM12625 AAP35599 AAI45321
REF NP_001091454 NP_001126995 NP_001244728 NP_002872 XP_001492315 XP_004656898 XP_005077223
SP P11234 Q4R379 Q5R4B8
TPG DAA32595