BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15768

Title: Backbone Resonance Assignments of the N-terminal half of hepatitis core protein

Authors: Duvignaud, Jean-Baptiste; Savard, Christian; Fromentin, Remi; Majeau, Nathalie; Leclerc, Denis; Gagne, Stephane

Citation: Duvignaud, Jean-Baptiste; Savard, Christian; Fromentin, Remi; Majeau, Nathalie; Leclerc, Denis; Gagne, Stephane. "Structure and dynamics of the N-terminal half of hepatitis C virus core protein: an intrinsically unstructured protein"  Biochem. Cell Biol. ., .-..

Assembly members:
HCV_core_protein, polymer, 88 residues, Formula weight is not available

Natural source:   Common Name: Hepatitis C virus   Taxonomy ID: 11103   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HCV_core_protein: MSTNPKPQRKTKRNTNRRPQ DVKFPGGGQIVGGVYLLPRR GPRLGVRATRKTSERSQPRG RRQPIPKARRPEGRTWAQPG YPHHHHHH

Data sets:
Data typeCount
13C chemical shifts157
15N chemical shifts48
1H chemical shifts48

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal half1

Entities:

Entity 1, N-terminal half 88 residues - Formula weight is not available

1   METSERTHRASNPROLYSPROGLNARGLYS
2   THRLYSARGASNTHRASNARGARGPROGLN
3   ASPVALLYSPHEPROGLYGLYGLYGLNILE
4   VALGLYGLYVALTYRLEULEUPROARGARG
5   GLYPROARGLEUGLYVALARGALATHRARG
6   LYSTHRSERGLUARGSERGLNPROARGGLY
7   ARGARGGLNPROILEPROLYSALAARGARG
8   PROGLUGLYARGTHRTRPALAGLNPROGLY
9   TYRPROHISHISHISHISHISHIS

Samples:

sample_1: HCV core protein, [U-100% 13C; U-100% 15N], 0.4 mM; TFE 90%; H2O 10%; DSS 0.2 mM; NaN3 0.1%

sample_conditions_1: pH: 6.6; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRView v5.2.2.01, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15767 4669
PDB
DBJ BAA00452 BAA00705 BAA00706 BAA00968 BAA01529
EMBL CAA03854 CAA43788 CAA43790 CAA43793 CAA46717
GB AAA16300 AAA16311 AAA20153 AAA20154 AAA21040
PIR JQ1926
REF NP_671491 YP_001469632
SP O39929 O92972 P26662 P26663 P26664