BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15708

Title: Structure of the N-terminal domain of the Y4 receptor in SDS and DPC micelles   PubMed: 18767100

Authors: Zou, Chao; Kumaran, Sowmini; Reto, Walser; Markovic, Stefan; Zerbe, Oliver

Citation: Zou, Chao; Kumaran, Sowmini; Markovic, Stefan; Walser, Reto; Zerbe, Oliver. "Studies of the Structure of the N-Terminal Domain from the Y4 Receptor-a G Protein-Coupled Receptor-and its Interaction with Hormones from the NPY Family"  ChemBioChem 9, 2276-2284 (2008).

Assembly members:
N-terminal_domain_of_Y4_receptor, polymer, 41 residues, 4561.191 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
N-terminal_domain_of_Y4_receptor: MNTSHLLALLLPKSPQGENR SKPLGTPYNFSEHCQDSVDV M

Data typeCount
1H chemical shifts537
heteronuclear NOE values67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 41 residues - 4561.191 Da.

1   METASNTHRSERHISLEULEUALALEULEU
2   LEUPROLYSSERPROGLNGLYGLUASNARG
3   SERLYSPROLEUGLYTHRPROTYRASNPHE
4   SERGLUHISCYSGLNASPSERVALASPVAL
5   MET

Samples:

sample_1: N-terminal domain of Y4 receptor 1 mM; MES 40 mM; SDS 300 mM

sample_2: N-terminal domain of Y4 receptor 1 mM; MES 40 mM; DPC 300 mM

sample_conditions_1: ionic strength: 0 M; pH: 5.6; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1

Software:

XEASY/CARA v1.5, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 15921 18319
DBJ BAG74162
EMBL CAA91433 CAG46748 CAI13318
GB AAB07759 AAC50280 AAH96238 AAH99637 AAP23199
REF NP_001265723 NP_001265724 NP_005963 XP_003804406 XP_003804407
SP P50391