BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15703

Title: Soluble form of human earliest activation antigen of leukocytes CD69   PubMed: 18959746

Authors: Kubickova, Monika; Kaderavek, Pavel; Chmelik, Josef; Zidek, Lukas; Sklenar, Vladimir

Citation: Vanek, Ondrej; Nalezkova, Monika; Kavan, Daniel; Borovickova, Ivana; Pompach, Petr; Novak, Petr; Kumar, Vinay; Vannucci, Luca; Hudecek, Jiri; Hofbauerova, Katerina; Kopecky, Vladimir; Brynda, Jiri; Kolenko, Petr; Dohnalek, Jan; Kaderavek, Pavel; Chmelik, Josef; Gorcik, Lukas; Zidek, Lukas; Sklenar, Vladimir; Bezouska, Karel. "Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice"  FEBS J. 275, 5589-5606 (2008).

Assembly members:
CD69, polymer, 130 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CD69: GQYTFSMPSDSHVSSCSEDW VGYQRKCYFISTVKRSWTSA QNACSEHGATLAVIDSEKDM NFLKRYAGREEHWVGLKKEP GHPWKWSNGKEFNNWFNVTG SDKCVFLKNTEVSSMECEKN LYWICNKPYK

Data sets:
Data typeCount
13C chemical shifts205
15N chemical shifts102
1H chemical shifts102
heteronuclear NOE values83
T1 relaxation values83
T2 relaxation values84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD691

Entities:

Entity 1, CD69 130 residues - Formula weight is not available

1   GLYGLNTYRTHRPHESERMETPROSERASP
2   SERHISVALSERSERCYSSERGLUASPTRP
3   VALGLYTYRGLNARGLYSCYSTYRPHEILE
4   SERTHRVALLYSARGSERTRPTHRSERALA
5   GLNASNALACYSSERGLUHISGLYALATHR
6   LEUALAVALILEASPSERGLULYSASPMET
7   ASNPHELEULYSARGTYRALAGLYARGGLU
8   GLUHISTRPVALGLYLEULYSLYSGLUPRO
9   GLYHISPROTRPLYSTRPSERASNGLYLYS
10   GLUPHEASNASNTRPPHEASNVALTHRGLY
11   SERASPLYSCYSVALPHELEULYSASNTHR
12   GLUVALSERSERMETGLUCYSGLULYSASN
13   LEUTYRTRPILECYSASNLYSPROTYRLYS

Samples:

sample_1: CD69, [U-13C; U-15N], 0.9 mM; sodium azide 1 mM; D2O, [U-99% 2H], 10%; sodium chloride 50 mM; MES 10 mM

sample_2: CD69, [U-15N], 0.65 mM; sodium azide 1 mM; D2O, [U-99% 2H], 10%; sodium chloride 50 mM; MES 10 mM

sample_conditions_1: ionic strength: 60 mM; pH: 5.8; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
T1sample_2isotropicsample_conditions_1
T2sample_2isotropicsample_conditions_1
ssNOEsample_2isotropicsample_conditions_1

Software:

SPARKY v3.111, Goddard - data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

Relax v1.2.6, d'Auvergne - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAF84558 BAG64271
EMBL CAA80298 CAA83017
GB AAB46359 AAH07037 AAO63584 ABM84101 ABM87473
REF NP_001772 XP_002822933 XP_003265512 XP_003829696 XP_004052729
SP Q07108