BMRB Entry 15684

Title:
rat lipocalin 2
Deposition date:
2008-03-17
Original release date:
2009-03-17
Authors:
Fang, Zhang; Lili, Lou; Hongchang, Gao; Donghai, Lin
Citation:

Citation: Zhang, Fang; Guo, Chenyun; Lou, Lili; Lin, Donghai. "Backbone and side-chain 1H, 13C, 15N resonance assignments of rat lipocalin2"  Biomol. NMR Assignments 3, 95-97 (2009).
PubMed: 19636955

Assembly members:

Assembly members:
rat_lipocalin_2, polymer, 188 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet22b

Data sets:
Data typeCount
13C chemical shifts647
15N chemical shifts175
1H chemical shifts1168

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1whole1

Entities:

Entity 1, whole 188 residues - Formula weight is not available

1   GLYSERGLNASPSERTHRGLNASNLEUILE
2   PROALAPROPROLEUILESERVALPROLEU
3   GLNPROGLYPHETRPTHRGLUARGPHEGLN
4   GLYARGTRPPHEVALVALGLYLEUALAGLY
5   ASNALAVALGLNLYSGLUARGGLNSERARG
6   PHETHRMETTYRSERTHRILETYRGLULEU
7   GLNGLUASPASNSERTYRASNVALTHRSER
8   ILELEUVALARGGLYGLNGLYCYSARGTYR
9   TRPILEARGTHRPHEVALPROSERSERARG
10   PROGLYGLNPHETHRLEUGLYASNILEHIS
11   SERTYRPROGLNILEGLNSERTYRASPVAL
12   GLNVALALAASPTHRASPTYRASPGLNPHE
13   ALAMETVALPHEPHEGLNLYSTHRSERGLU
14   ASNLYSGLNTYRPHELYSVALTHRLEUTYR
15   GLYARGTHRLYSGLYLEUSERASPGLULEU
16   LYSGLUARGPHEVALSERPHEALALYSSER
17   LEUGLYLEULYSASPASNASNILEVALPHE
18   SERVALPROTHRASPGLNCYSILEASPASN
19   LEUGLUHISHISHISHISHISHIS

Samples:

sample_1: rat lipocalin 2, [U-99% 13C; U-99% 15N], 0.8 mM

sample_conditions_1: ionic strength: 0.17 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAA31657
GB AAB35994 AAH89053 EDL93236
REF NP_570097
SP P30152
AlphaFold P30152

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks