BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15624

Title: Structure of Calmodulin complexed with the Calmodulin Binding Domain of Calcineurin

Authors: Chyan, Chia-Lin; Irene, Deli

Citation: Chyan, Chia-Lin; Irene, Deli. "Structure of Calmodulin complexed with the Calmodulin Binding Domain of Calcineurin"  Not known ., .-..

Assembly members:
Calmodulin, polymer, 148 residues, Formula weight is not available
Calmodulin_binding_domain_of_Calcineurin, polymer, 24 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
Calmodulin_binding_domain_of_Calcineurin: ARKEVIRNKIRAIGKMARVF SVLR

Data sets:
Data typeCount
13C chemical shifts696
15N chemical shifts179
1H chemical shifts1151

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22
3CALCIUM ION3

Entities:

Entity 1, entity_1 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, entity_2 24 residues - Formula weight is not available

1   ALAARGLYSGLUVALILEARGASNLYSILE
2   ARGALAILEGLYLYSMETALAARGVALPHE
3   SERVALLEUARG

Entity 3, CALCIUM ION - Ca - 40.078 Da.

1   CA

Samples:

CaMCaNp: Calmodulin, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; Calmodulin binding domain of Calcineurin, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; CALCIUM ION 1 ± 0.05 mM

sample_conditions_1: pH: 6.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCCaMCaNpisotropicsample_conditions_1
2D 1H-13C HSQCCaMCaNpisotropicsample_conditions_1
3D HNCACaMCaNpisotropicsample_conditions_1
3D HN(CO)CACaMCaNpisotropicsample_conditions_1
3D CBCA(CO)NHCaMCaNpisotropicsample_conditions_1
3D CBCANHCaMCaNpisotropicsample_conditions_1
3D C(CO)NHCaMCaNpisotropicsample_conditions_1
3D HBHA(CO)NHCaMCaNpisotropicsample_conditions_1
3D HNCOCaMCaNpisotropicsample_conditions_1
3D HCCH TOCSYCaMCaNpisotropicsample_conditions_1
3D 1H-15N NOESYCaMCaNpisotropicsample_conditions_1
3D 1H-13C NOESYCaMCaNpisotropicsample_conditions_1
2D DQF-COSYCaMCaNpisotropicsample_conditions_1
2D 1H-1H TOCSYCaMCaNpisotropicsample_conditions_1
2D 1H-1H NOESYCaMCaNpisotropicsample_conditions_1
3D H(CCO)NHCaMCaNpisotropicsample_conditions_1

Software:

CYANA v2.1, Herrmann, Guntert and Wuthrich - automated NOEs assignment, structure calculation

AURELIA v3.6, Neidig, Geyer, Gorler, Antz, Saffrich, Beneicke, Kalbitzer - peak picking, processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PIR P62158 JC1305 MCON
BMRB 15184 15185 15186 15187 15188 15191 15470 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4270 4284 4310 6023
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 BAA14083 BAB19673 BAC36398 BAE27131 BAE29521
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 CAA40398 CAA54807 CAB89253 CAF94324 CAH91746
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 AAA02631 AAA35634 AAA37359 AAA37432 AAA40940
PRF 0409298A 0608335A 1714202A 2006264A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_000935 NP_001074063 NP_001076161 NP_001080813 NP_001087372
SP O02367 O16305 O96081 P02594 P05932 P48452 P63328 P63329 Q08209 Q27889
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 DAA28842