BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15622

Title: Solution structure of an antilipopolysaccharide factor from shrimp   PubMed: 18466970

Authors: Yang, Yinshan; Boze, Helene; Chemardin, Patrick; Padilla, Andre; Moulin, Guy; Tassanakajon, Anchalaee; Pugniere, Martine; Roquet, Francoise; Gueguen, Yannick; Bachere, Evelyne; Aumelas, Andre

Citation: Somboonwiwat, Kunlaya; Bachere, Evelyne; Rimphanitchayakit, Vichien; Tassanakajon, Anchalaee. "Localization of anti-lipopolysaccharide factor (ALFPm3) in tissues of the black tiger shrimp, Penaeus monodon, and characterization of its binding properties."  Dev. Comp. Immunol. 32, 1170-1176 (2008).

Assembly members:
antilipopolysaccharide_factor, polymer, 102 residues, 11534.318 Da.

Natural source:   Common Name: black tiger shrimp   Taxonomy ID: 6687   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Penaeus monodon

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris

Entity Sequences (FASTA):
antilipopolysaccharide_factor: EAYVQGWEAVAAAVASKIVG LWRNEKTELLGHECKFTVKP YLKRFQVYYKGRMWCPGWTA IRGEASTRSQSGVAGKTAKD FVRKAFQKGLISQQEANQWL SS

Data sets:
Data typeCount
15N chemical shifts90
1H chemical shifts619

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1antilipopolysaccharide factor1

Entities:

Entity 1, antilipopolysaccharide factor 102 residues - 11534.318 Da.

1   GLUALATYRVALGLNGLYTRPGLUALAVAL
2   ALAALAALAVALALASERLYSILEVALGLY
3   LEUTRPARGASNGLULYSTHRGLULEULEU
4   GLYHISGLUCYSLYSPHETHRVALLYSPRO
5   TYRLEULYSARGPHEGLNVALTYRTYRLYS
6   GLYARGMETTRPCYSPROGLYTRPTHRALA
7   ILEARGGLYGLUALASERTHRARGSERGLN
8   SERGLYVALALAGLYLYSTHRALALYSASP
9   PHEVALARGLYSALAPHEGLNLYSGLYLEU
10   ILESERGLNGLNGLUALAASNGLNTRPLEU
11   SERSER

Samples:

sample_1: ALF 1 mM

sample_2: ALF, [U-99% 15N], 1 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.9; pressure: 1 atm; temperature: 305 K

sample_conditions_2: ionic strength: 0 M; pH: 6.9; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
3D 1H-15N HSQC-NOESYsample_2isotropicsample_conditions_2

Software:

xwinnmr, Bruker Biospin - collection

GIFA, Delsuc - processing

X-PLOR, Brunger - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB ABP73289 ABP73292 ACC86067 ADC32520 ADK74771