BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15576

Title: Ubiquitin-like domain of NFATC2IP. Northeast Structural Genomics Consortium Target hr5627.

Authors: Doherty, Ryan; Dhe-Paganon, Sirano; Fares, Christophe; Lemak, Alex; Butler, Chris; Srisailam, Sampath; Karra, Murthy; Yee, Adelinda; Edwards, Aled; Weigelt, Johan; Arrowsmith, Cheryl

Citation: Doherty, Ryan; Dhe-Paganon, Sirano; Fares, Christophe; Lemak, Alex; Butler, Chris; Srisailam, Sampath; Karra, Murthy; Yee, Adelinda; Weigelt, Johan; Edwards, Aled; Arrowsmith, Cheryl. "Solution structure of Ubiquitin-like domain of NFATC2IP."  The BMRB entry is the only known published source for the data..

Assembly members:
NFATC2IP, polymer, 97 residues, 8739.932 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NFATC2IP: MGSSHHHHHHSSGLVPRGST ETSQQLQLRVQGKEKHQTLE VSLSRDSPLKTLMSHYEEAM GLSGRKLSFFFDGTKLSGRE LPADLGMESGDLIEVWG

Data sets:
Data typeCount
13C chemical shifts312
15N chemical shifts71
1H chemical shifts512

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NFATC2IP1

Entities:

Entity 1, NFATC2IP 97 residues - 8739.932 Da.

Residues 1-19 represent a non-native affinity tag.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERTHR
3   GLUTHRSERGLNGLNLEUGLNLEUARGVAL
4   GLNGLYLYSGLULYSHISGLNTHRLEUGLU
5   VALSERLEUSERARGASPSERPROLEULYS
6   THRLEUMETSERHISTYRGLUGLUALAMET
7   GLYLEUSERGLYARGLYSLEUSERPHEPHE
8   PHEASPGLYTHRLYSLEUSERGLYARGGLU
9   LEUPROALAASPLEUGLYMETGLUSERGLY
10   ASPLEUILEGLUVALTRPGLY

Samples:

H2O_NH: NFATC2IP, [U-15N], 0.9 mM; NaCl 500 mM; Tris-HCl 50 mM; Benzamidine 1 mM; NaN3 0.01%; ZnSO4 0.01 mM; D2O, [U-100% 2H], 10%; H2O 90%

D2O_CN: NFATC2IP, [U-13C; U-15N], 0.9 mM; NaCl 500 mM; Tris-HCl 50 mM; Benzamidine 1 mM; NaN3 0.01%; ZnSO4 0.01 mM; D2O, [U-100% 2H], 100%

H2O_CN: NFATC2IP, [U-13C; U-15N], 0.9 mM; NaCl 500 mM; Tris-HCl 50 mM; Benzamidine 1 mM; NaN3 0.01%; ZnSO4 0.01 mM; D2O, [U-100% 2H], 10%; H2O 90%

H2O_CN_phage: NFATC2IP, [U-13C; U-15N], 0.9 mM; NaCl 500 mM; Tris-HCl 50 mM; Benzamidine 1 mM; NaN3 0.01%; ZnSO4 0.01 mM; Pf1 co-solvent Protease free Phage 12 mg/ml; D2O, [U-100% 2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 500 mM; pH: 8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCH2O_NHisotropicsample_conditions_1
2D 1H-13C Constant Time HSQCD2O_CNisotropicsample_conditions_1
3D HNCOH2O_CNisotropicsample_conditions_1
3D HNCAH2O_CNisotropicsample_conditions_1
3D CBCA(CO)NHH2O_CNisotropicsample_conditions_1
3D HBHA(CO)NHH2O_CNisotropicsample_conditions_1
3D 1H-13C NOESYH2O_CNisotropicsample_conditions_1
3D 1H-13C Aromatic NOESYH2O_CNisotropicsample_conditions_1
3D (H)CCH-TOCSYD2O_CNisotropicsample_conditions_1
3D H(C)CH-TOCSYD2O_CNisotropicsample_conditions_1
2D 1H-15N HSQCH2O_CNisotropicsample_conditions_1
2D 1H-15N IPAP HSQCH2O_CNisotropicsample_conditions_1
2D 1H-15N IPAP HSQCH2O_CN_phageanisotropicsample_conditions_1
3D 1H-15N NOESYH2O_CNisotropicsample_conditions_1
3D 1H-13C NOESYD2O_CNisotropicsample_conditions_1

Software:

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

SPARKY, Goddard - peak picking

TOPSPIN, Bruker Biospin - collection

PSVS, Bhattacharya and Montelione - refinement

WhatIF, Vriend - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PIR Q8NCF5
REF NP_116204 NP_116204 XP_001102777 XP_001146533 XP_002802482 XP_002826318
PDB
DBJ BAB17279 BAB55189 BAC11189 BAH12551 BAH14223
GB AAH18311 AAH21551 AAH68007 AAH80628 AAI01742
SP Q8NCF5 Q9GLZ9