BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15555

Title: NMR structure of human Serine protease inhibitor Kazal type II (SPINK2)   PubMed: 19422058

Authors: Ting, Chen; Tian-Ren, Lee; Ping-Chiang, Lyu

Citation: Chen, Ting; Lee, Tian-Ren; Liang, Wei-Guang; Chang, Wun-Shaing Wayne; Lyu, Ping-Chiang. "Identification of trypsin-inhibitory site and structure determination of human SPINK2 serine proteinase inhibitor"  Proteins 77, 209-219 (2009).

Assembly members:
SPINK2, polymer, 62 residues, 7025.208 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SPINK2: PQFGLFSKYRTPNCSQYRLP GCPRHFNPVCGSDMSTYANE CTLCMKIREGGHNIKIIRNG PC

Data sets:
Data typeCount
1H chemical shifts345

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SPINK21

Entities:

Entity 1, SPINK2 62 residues - 7025.208 Da.

1   PROGLNPHEGLYLEUPHESERLYSTYRARG
2   THRPROASNCYSSERGLNTYRARGLEUPRO
3   GLYCYSPROARGHISPHEASNPROVALCYS
4   GLYSERASPMETSERTHRTYRALAASNGLU
5   CYSTHRLEUCYSMETLYSILEARGGLUGLY
6   GLYHISASNILELYSILEILEARGASNGLY
7   PROCYS

Samples:

sample: SPINK2 1.5 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 3.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsampleanisotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
DBJ BAG35155
EMBL CAB37834 CAG46932
GB AAB59431 AAH22514 ABM86459 ABW03650 ADU87624
PRF 1616228A
REF NP_001258647 NP_001258649 NP_066937 XP_001136807 XP_001136960
SP P20155