BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15550

Title: Solution structure of hPCIF1 WW domain

Authors: Kouno, Takahide; Iwamoto, Yu; Hirose, Yutaka; Aizawa, Tomoyasu; Demura, Makoto; Kawano, Keiichi; Ohkuma, Yoshiaki; Mizuguchi, Mineyuki

Citation: Kouno, Takahide; Iwamoto, Yu; Hirose, Yutaka; Aizawa, Tomoyasu; Demura, Makoto; Kawano, Keiichi; Ohkuma, Yoshiaki; Mizuguchi, Mineyuki. "1H, 13C, and 15N resonance assignments of hPCIF1 WW domain"  Not known ., .-..

Assembly members:
hPCIF1_WW_domain, polymer, 47 residues, 5743.482 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
hPCIF1_WW_domain: LPEELVHAGWEKCWSRRENR PYYFNRFTNQSLWEMPVLGQ HDVISDP

Data sets:
Data typeCount
13C chemical shifts187
15N chemical shifts52
1H chemical shifts336

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chain A1

Entities:

Entity 1, chain A 47 residues - 5743.482 Da.

1   LEUPROGLUGLULEUVALHISALAGLYTRP
2   GLULYSCYSTRPSERARGARGGLUASNARG
3   PROTYRTYRPHEASNARGPHETHRASNGLN
4   SERLEUTRPGLUMETPROVALLEUGLYGLN
5   HISASPVALILESERASPPRO

Samples:

sample_1: hPCIF1 WW domain, [U-15N], 0.6 mM; sodium phosphate 20 mM; sodium chloride 20 mM; DTT, [U-2H], 1 mM; sodium azide 1 mM

sample_2: hPCIF1 WW domain, [U-13C; U-15N], 0.6 mM; sodium phosphate 20 mM; sodium chloride 20 mM; DTT, [U-2H], 1 mM; sodium azide 1 mM

sample_3: hPCIF1 WW domain 0.6 mM; sodium phosphate 20 mM; sodium chloride 20 mM; DTT, [U-2H], 1 mM; sodium azide 1 mM

sample_4: hPCIF1 WW domain, [U-13C; U-15N], 0.6 mM; sodium phosphate 20 mM; sodium chloride 20 mM; DTT, [U-2H], 1 mM; sodium azide 1 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1
3D HNCAsample_4isotropicsample_conditions_1
3D CBCA(CO)NHsample_4isotropicsample_conditions_1
3D C(CO)NHsample_4isotropicsample_conditions_1
3D HBHA(CO)NHsample_4isotropicsample_conditions_1
3D HNCOsample_4isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_3isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP, Garrett - collection

X-PLOR, Brunger - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAC45238 BAG11416 BAH13031
EMBL CAH93090
GB AAH10005 AAH13365 AAI49976 ACE87073 ACE87754
PIR JC7932
REF NP_001096569 NP_001126830 NP_071387 XP_001105396 XP_001503426
SP Q9H4Z3
TPG DAA23280