BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15546

Title: Chemical Shift Assignment of the Transmembrane Helices of DsbB by 3D Magic Angle Spinning NMR   PubMed: 18227427

Authors: Li, Ying; Berthold, Deborah; Frericks-Schmidt, Heather; Gennis, Robert; Rienstra, Chad

Citation: Li, Ying; Berthold, Deborah; Gennis, Robert; Rienstra, Chad. "Chemical shift assignment of the transmembrane helices of DsbB, a 20-kDa integral membrane enzyme, by 3D magic-angle spinning NMR spectroscopy"  Protein Sci. 17, 199-204 (2008).

Assembly members:
DsbB, polymer, 186 residues, Formula weight is not available
UQ8, non-polymer, 250.290 Da.

Natural source:   Common Name: not available   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DsbB: MLRFLNQASQGRGAWLLMAF TALALELTALWFQHVMLLKP SVLCIYERVALFGVLGAALI GAIAPKTPLRYVAMVIWLYS AFRGVQLTYEHTMLQLYPSP FATCDFMVRFPEWLPLDKWV PQVFVASGDCAERQWDFLGL EMPQWLLGIFIAYLIVAVLV VISQPFKAKKRDLFGRGSRS HHHHHH

Data sets:
Data typeCount
13C chemical shifts201
15N chemical shifts57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DsbB ploypeptide1
2substrate2

Entities:

Entity 1, DsbB ploypeptide 186 residues - Formula weight is not available

1   METLEUARGPHELEUASNGLNALASERGLN
2   GLYARGGLYALATRPLEULEUMETALAPHE
3   THRALALEUALALEUGLULEUTHRALALEU
4   TRPPHEGLNHISVALMETLEULEULYSPRO
5   SERVALLEUCYSILETYRGLUARGVALALA
6   LEUPHEGLYVALLEUGLYALAALALEUILE
7   GLYALAILEALAPROLYSTHRPROLEUARG
8   TYRVALALAMETVALILETRPLEUTYRSER
9   ALAPHEARGGLYVALGLNLEUTHRTYRGLU
10   HISTHRMETLEUGLNLEUTYRPROSERPRO
11   PHEALATHRCYSASPPHEMETVALARGPHE
12   PROGLUTRPLEUPROLEUASPLYSTRPVAL
13   PROGLNVALPHEVALALASERGLYASPCYS
14   ALAGLUARGGLNTRPASPPHELEUGLYLEU
15   GLUMETPROGLNTRPLEULEUGLYILEPHE
16   ILEALATYRLEUILEVALALAVALLEUVAL
17   VALILESERGLNPROPHELYSALALYSLYS
18   ARGASPLEUPHEGLYARGGLYSERARGSER
19   HISHISHISHISHISHIS

Entity 2, substrate - C14 H18 O4 - 250.290 Da.

1   UQ8

Samples:

sample_1: DsbB, [U-100% 13C; U-100% 15N], 20 mg

sample_conditions_1: ionic strength: 25 mM; pH: 7.5; pressure: 1 atm; temperature controller setting: 223 K

Experiments:

NameSampleSample stateSample conditions
2D CC DARR mixingsample_1solidsample_conditions_1
3D NCACXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
3D CAN(CO)CXsample_1solidsample_conditions_1
3D CON(CA)CXsample_1solidsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

BMRB 18395 18493
PDB
DBJ BAA36032 BAB35103 BAG76757 BAI24997 BAI30121
EMBL CAP75720 CAQ31687 CAQ98064 CAR02574 CAR12682
GB AAA23711 AAB25233 AAC74269 AAG56036 AAN42789
PIR H85696
REF NP_309707 NP_415703 NP_707082 WP_000652474 WP_000943442
SP A1AAA8 P0A6M2 P0A6M3 P59343 Q0T5L6