BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15533

Title: Structure of the Wilms' Tumor Suppressor Protein Zinc Finger Domain Bound to DNA   PubMed: 17716689

Authors: Stoll, Raphael; Lee, Brian; Debler, Erik; Laity, John; Wilson, Ian; Dyson, Helen; Wright, Peter

Citation: Stoll, Raphael; Lee, Brian; Debler, Erik; Laity, John; Wilson, Ian; Dyson, Helen; Wright, Peter. "Structure of the Wilms Tumor Suppressor Protein Zinc Finger Domain Bound to DNA"  J. Mol. Biol. 372, 1227-1245 (2007).

Assembly members:
wt1-zf14, polymer, 119 residues, 14494.849 Da.
ZN, non-polymer, 65.409 Da.
wt1-14mer_1, polymer, 14 residues, Formula weight is not available
wt1-14mer_2, polymer, 14 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
wt1-zf14: ASEKRPFMCAYPGCNKRYFK LSHLQMHSRKHTGEKPYQCD FKDCERRFSRSDQLKRHQRR HTGVKPFQCKTCQRKFSRSD HLKTHTRTHTGEKPFSCRWP SCQKKFARSDELVRHHNMH
wt1-14mer_1: CGCGGGGGCGTCTG
wt1-14mer_2: CAGACGCCCCCGCG

Data sets:
Data typeCount
15N chemical shifts115
1H chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1wt1-zf141
2zinc12
3zinc22
4zinc32
5zinc42
6strand13
7strand24

Entities:

Entity 1, wt1-zf14 119 residues - 14494.849 Da.

1   ALASERGLULYSARGPROPHEMETCYSALA
2   TYRPROGLYCYSASNLYSARGTYRPHELYS
3   LEUSERHISLEUGLNMETHISSERARGLYS
4   HISTHRGLYGLULYSPROTYRGLNCYSASP
5   PHELYSASPCYSGLUARGARGPHESERARG
6   SERASPGLNLEULYSARGHISGLNARGARG
7   HISTHRGLYVALLYSPROPHEGLNCYSLYS
8   THRCYSGLNARGLYSPHESERARGSERASP
9   HISLEULYSTHRHISTHRARGTHRHISTHR
10   GLYGLULYSPROPHESERCYSARGTRPPRO
11   SERCYSGLNLYSLYSPHEALAARGSERASP
12   GLULEUVALARGHISHISASNMETHIS

Entity 2, zinc1 - Zn - 65.409 Da.

1   ZN

Entity 3, strand1 14 residues - Formula weight is not available

1   DCDGDCDGDGDGDGDGDCDG
2   DTDCDTDG

Entity 4, strand2 14 residues - Formula weight is not available

1   DCDADGDADCDGDCDCDCDC
2   DCDGDCDG

Samples:

sample_1: wt1-14mer, [U-100% 13C; U-100% 15N], 0.6 mM; Tris/HCl 10 mM; KCl 20 mM; ZnSO4 5 uM; NaN3 2 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.7; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
IPAPsample_1anisotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Koll - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 15532 4707 4708 4709 4710
PDB
DBJ BAA11522 BAA28147 BAA94793 BAF84425
EMBL CAA35956 CAA43819 CDG23662
GB AAA36810 AAA61299 AAB33443 AAB53152 AAH32861
PRF 1604420A
REF NP_000369 NP_001001264 NP_001079057 NP_001079336 NP_001135625
SP B5DE03 O62651 P19544 P79958
TPG DAA21902