BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15518

Title: 1H, 15N, 13CO, 13Calpha and 13Cbeta Chemical Shift Assignments of Human Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain Bound to GW1929   PubMed: 17980149

Authors: Lu, Jianyun

Citation: Lu, Jianyun; Chen, Minghe; Stanley, Susan; Li, Ellen. "Effect of Heterodimer Partner RXRalpha on PPARgamma Activation Function-2 Helix in Solution"  Biochem. Biophys. Res. Commun. 365, 42-46 (2008).

Assembly members:
Peroxisome_Proliferator-Activated_Receptor_Gamma_Ligand-Binding_Domain, polymer, 279 residues, Formula weight is not available
GW1929, non-polymer, 495.57 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Peroxisome_Proliferator-Activated_Receptor_Gamma_Ligand-Binding_Domain: GSHMQLNPESADLRALAKHL YDSYIKSFPLTKAKARAILT GKTTDKSPFVIYDMNSLMMG EDKIKFKHITPLQEQSKEVA IRIFQGCQFRSVEAVQEITE YAKSIPGFVNLDLNDQVTLL KYGVHEIIYTMLASLMNKDG VLISEGQGFMTREFLKSLRK PFGDFMEPKFEFAVKFNALE LDDSDLAIFIAVIILSGDRP GLLNVKPIEDIQDNLLQALE LQLKLNHPESSQLFAKLLQK MTDLRQIVTEHVQLLQVIKK TETDMSLHPLLQEIYKDLY

Data sets:
Data typeCount
13C chemical shifts772
15N chemical shifts244
1H chemical shifts244

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PPARgamma LBD1
2GW19292

Entities:

Entity 1, PPARgamma LBD 279 residues - Formula weight is not available

1   GLYSERHISMETGLNLEUASNPROGLUSER
2   ALAASPLEUARGALALEUALALYSHISLEU
3   TYRASPSERTYRILELYSSERPHEPROLEU
4   THRLYSALALYSALAARGALAILELEUTHR
5   GLYLYSTHRTHRASPLYSSERPROPHEVAL
6   ILETYRASPMETASNSERLEUMETMETGLY
7   GLUASPLYSILELYSPHELYSHISILETHR
8   PROLEUGLNGLUGLNSERLYSGLUVALALA
9   ILEARGILEPHEGLNGLYCYSGLNPHEARG
10   SERVALGLUALAVALGLNGLUILETHRGLU
11   TYRALALYSSERILEPROGLYPHEVALASN
12   LEUASPLEUASNASPGLNVALTHRLEULEU
13   LYSTYRGLYVALHISGLUILEILETYRTHR
14   METLEUALASERLEUMETASNLYSASPGLY
15   VALLEUILESERGLUGLYGLNGLYPHEMET
16   THRARGGLUPHELEULYSSERLEUARGLYS
17   PROPHEGLYASPPHEMETGLUPROLYSPHE
18   GLUPHEALAVALLYSPHEASNALALEUGLU
19   LEUASPASPSERASPLEUALAILEPHEILE
20   ALAVALILEILELEUSERGLYASPARGPRO
21   GLYLEULEUASNVALLYSPROILEGLUASP
22   ILEGLNASPASNLEULEUGLNALALEUGLU
23   LEUGLNLEULYSLEUASNHISPROGLUSER
24   SERGLNLEUPHEALALYSLEULEUGLNLYS
25   METTHRASPLEUARGGLNILEVALTHRGLU
26   HISVALGLNLEULEUGLNVALILELYSLYS
27   THRGLUTHRASPMETSERLEUHISPROLEU
28   LEUGLNGLUILETYRLYSASPLEUTYR

Entity 2, GW1929 - C30 H29 N3 O4 - 495.57 Da.

1   GW1929

Samples:

sample_1: Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain, [U-13C; U-15N; U-2H], 1.0 mM; potassium phosphate 20 mM; KCl 50 mM; NaN3 0.05%; EDTA-d16 0.5 mM; b-mercaptoethanol-d6 8 mM; H20 95%; D2O 5%

sample_conditions_1: ionic strength: 0.103 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
4D HNCOCAsample_1isotropicsample_conditions_1
4D HNCOi-1CAisample_1isotropicsample_conditions_1
4D HNCACOsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 700 MHz

Related Database Links:

BMRB 17975 17976 17977
PDB
DBJ BAA18949 BAA23354 BAA32540 BAA36485 BAD20642
EMBL CAA07224 CAA07225 CAA62152 CAA62153 CAA73032
GB AAA19971 AAA62110 AAA62277 AAA80314 AAB04028
PIR JE0279
REF NP_001019803 NP_001028032 NP_001075617 NP_001094391 NP_001106647
SP O18924 O18971 O19052 O62807 O88275
TPG DAA16769