BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15512

Title: 1H, 13C, and 15N NMR chemical shift assignments of the palladin Ig3 domain   PubMed: 19636923

Authors: Dixon, Richard; Rachlin, Andrew; Otey, Carol; Campbell, Sharon

Citation: Dixon, Richard; Campbell, Sharon. "1H, 15N, and 13C NMR chemical shift assignments for the Ig3 domain of palladin"  Biomol. NMR Assignments 2, 51-53 (2008).

Assembly members:
Palladin_Ig3_domain, polymer, 108 residues, 11975 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Palladin_Ig3_domain: GGSNATAPFFEMKLKHYKIF EGMPVTFTCRVAGNPKPKIY WFKDGKQISPKSDHYTIQRD LDGTCSLHTTASTLDDDGNY TIMAANPQGRVSCTGRLMVQ AVNQRGRS

Data sets:
Data typeCount
13C chemical shifts455
15N chemical shifts112
1H chemical shifts701

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1molecule 11

Entities:

Entity 1, molecule 1 108 residues - 11975 Da.

Residues 1-3 are non-native amino acids resulting from the cloning and purification strategy

1   GLYGLYSERASNALATHRALAPROPHEPHE
2   GLUMETLYSLEULYSHISTYRLYSILEPHE
3   GLUGLYMETPROVALTHRPHETHRCYSARG
4   VALALAGLYASNPROLYSPROLYSILETYR
5   TRPPHELYSASPGLYLYSGLNILESERPRO
6   LYSSERASPHISTYRTHRILEGLNARGASP
7   LEUASPGLYTHRCYSSERLEUHISTHRTHR
8   ALASERTHRLEUASPASPASPGLYASNTYR
9   THRILEMETALAALAASNPROGLNGLYARG
10   VALSERCYSTHRGLYARGLEUMETVALGLN
11   ALAVALASNGLNARGGLYARGSER

Samples:

sample_1: Palladin Ig3 domain, [U-98% 13C; U-98% 15N], 0.3-1.8 mM; D2O 10%; H2O 90%; TRIS 50 mM; sodium chloride 150 mM; DTT 2 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC (aliphatic)sample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D C(CO)NH TOCSYsample_1isotropicsample_conditions_1
3D HC(CO)NH TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY (aliphatic)sample_1isotropicsample_conditions_1
HBCBCGCDHDsample_1isotropicsample_conditions_1
HBCBCGCDCEHEsample_1isotropicsample_conditions_1
2D 1H-13C HSQC (aromatic)sample_1isotropicsample_conditions_1
3D 1H-13C NOESY (aromatic)sample_1isotropicsample_conditions_1

Software:

NMRView v5.2.2.01, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 700 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB EDL28653 EDL28654