BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15490

Title: Solution Structure of E coli NusG carboxyterminal domain   PubMed: 19500594

Authors: Schweimer, Kristian; Scheckenhofer, Ulrich; Roesch, Paul

Citation: Mooney, Rachel Anne; Schweimer, Kristian; Roesch, Paul; Gottesman, Max; Landick, Robert. "Two structurally independent domains of E. coli NusG create regulatory plasticity via distinct interactions with RNA polymerase and regulators."  J. Mol. Biol. 391, 341-358 (2009).

Assembly members:
NusG, polymer, 181 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NusG: MSEAPKKRWYVVQAFSGFEG RVATSLREHIKLHNMEDLFG EVMVPTEEVVEIRGGQRRKS ERKFFPGYVLVQMVMNDASW HLVRSVPRVMGFIGGTSDRP APISDKEVDAIMNRLQQVGD KPRPKTLFEPGEMVRVNDGP FADFNGVVEEVDYEKSRLKV SVSIFGRATPVELDFSQVEK A

Data sets:
Data typeCount
13C chemical shifts254
15N chemical shifts54
1H chemical shifts400

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NusG1

Entities:

Entity 1, NusG 181 residues - Formula weight is not available

1   METSERGLUALAPROLYSLYSARGTRPTYR
2   VALVALGLNALAPHESERGLYPHEGLUGLY
3   ARGVALALATHRSERLEUARGGLUHISILE
4   LYSLEUHISASNMETGLUASPLEUPHEGLY
5   GLUVALMETVALPROTHRGLUGLUVALVAL
6   GLUILEARGGLYGLYGLNARGARGLYSSER
7   GLUARGLYSPHEPHEPROGLYTYRVALLEU
8   VALGLNMETVALMETASNASPALASERTRP
9   HISLEUVALARGSERVALPROARGVALMET
10   GLYPHEILEGLYGLYTHRSERASPARGPRO
11   ALAPROILESERASPLYSGLUVALASPALA
12   ILEMETASNARGLEUGLNGLNVALGLYASP
13   LYSPROARGPROLYSTHRLEUPHEGLUPRO
14   GLYGLUMETVALARGVALASNASPGLYPRO
15   PHEALAASPPHEASNGLYVALVALGLUGLU
16   VALASPTYRGLULYSSERARGLEULYSVAL
17   SERVALSERILEPHEGLYARGALATHRPRO
18   VALGLULEUASPPHESERGLNVALGLULYS
19   ALA

Samples:

sample_1: NusG, [U-95% 13C; U-95% 15N], 0.45 mM; potassium phosphate 10 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

X-PLOR NIH v1.2.1, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 15642
PDB
DBJ BAB38328 BAE77338 BAG79793 BAH61119 BAI28242
EMBL CAD09492 CAG73137 CAO95196 CAP78438 CAQ34328
GB AAA24622 AAC43080 AAC76956 AAF33495 AAG59178
PIR AB0934
REF NP_312932 NP_418409 NP_457922 NP_463017 NP_709777
SP P0AA01 P0AA02 P0AA03 P0AFG0 P0AFG1