BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15455

Title: AbaA3-DKP-insulin   PubMed: 17884811

Authors: Huang, Kun; Chan, Shu; Hua, Qing-xin; Chu, Ying-chi; Wang, Run-ying; Klaproth, Birgit; Jia, Wenhua; Whittaker, Jonathan; De Meyts, Pierre; Nakagawa, Satoe; Steiner, Donald; Katsoyannis, Panayotis; Weiss, Michael

Citation: Huang, Kun; Chan, Shu; Hua, Qing-xin; Chu, Ying-chi; Wang, Run-ying; Klaproth, Birgit; Jia, Wenhua; Whittaker, Jonathan; De Meyts, Pierre; Nakagawa, Satoe; Steiner, Donald; Katsoyannis, Panayotis; Weiss, Michael. "The A-chain of insulin contacts the insert domain of the insulin receptor. Photo-cross-linking and mutagenesis of a diabetes-related crevice"  J. Biol. Chem. 282, 35337-35349 (2007).

Assembly members:
A_chain, polymer, 21 residues, 2294.606 Da.
B_chain, polymer, 30 residues, 3411.928 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
A_chain: GIXEQCCTSICSLYQLENYC N
B_chain: FVNQHLCGSDLVEALYLVCG ERGFFYTKPT

Data sets:
Data typeCount
1H chemical shifts301

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 21 residues - 2294.606 Da.

1   GLYILEABAGLUGLNCYSCYSTHRSERILE
2   CYSSERLEUTYRGLNLEUGLUASNTYRCYS
3   ASN

Entity 2, entity_2 30 residues - 3411.928 Da.

1   PHEVALASNGLNHISLEUCYSGLYSERASP
2   LEUVALGLUALALEUTYRLEUVALCYSGLY
3   GLUARGGLYPHEPHETYRTHRLYSPROTHR

Samples:

sample_1: AbaA3-DKP-insulin 1 mM; H2O 90%; D2O 10%

sample_2: AbaA3-DKP-insulin 1 mM; D2O 10%

sample_3: AbaA3-DKP-insulin 1 mM; H2O 80%; D-acetic acid 20%

sample_4: AbaA3-DKP-insulin 1 mM; D2O 80%; D-acetic acid 20%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 308 K

sample_conditions_2: pD: 6.6; pressure: 1 atm; temperature: 308 K

sample_conditions_3: pD: 7.6; pressure: 1 atm; temperature: 315 K

sample_conditions_4: pH*: 1.9; pressure: 1 atm; temperature: 308 K

sample_conditions_5: pD: 1.9; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_2
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-1H COSYsample_3isotropicsample_conditions_3
2D 1H-1H TOCSYsample_3isotropicsample_conditions_3
2D 1H-1H NOESYsample_3isotropicsample_conditions_3
2D DQF-COSYsample_4isotropicsample_conditions_4
2D 1H-1H TOCSYsample_4isotropicsample_conditions_4
2D 1H-1H NOESYsample_4isotropicsample_conditions_4
2D DQF-COSYsample_4isotropicsample_conditions_5
2D 1H-1H TOCSYsample_4isotropicsample_conditions_5
2D 1H-1H NOESYsample_4isotropicsample_conditions_5

Software:

CNS, Accelrys Software Inc. - refinement

X-PLOR v3.1, Brunger - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker DMX 800 MHz

Related Database Links:

BMRB 15454 15450 15454 16608 17108 1760 1762 5246
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