BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15451

Title: GABPa OST domain   PubMed: 18295234

Authors: Kang, Hyun-Seo; Nelson, Mary; Mackereth, Cameron; Schaerpf, Manuela; Graves, Barbara; McIntosh, Lawrence

Citation: Kang, Hyun-Seo; Nelson, Mary; Mackereth, Cameron; Schaerpf, Manuela; Graves, Barbara; McIntosh, Lawrence. "Identification and structural characterization of a CBP/p300-binding domain from the ETS family transcription factor GABP alpha"  J. Mol. Biol. 377, 636-646 (2008).

Assembly members:
OST_(On-SighT), polymer, 89 residues, 9979.494 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
OST_(On-SighT): MAECVSQAIDINEPIGNLKK LLEPRLQCSLDAHEICLQDI QLDPDRSLFDQGVKTDGTVQ LSVQVISYQGMEPKLNILEI VKTAETVEW

Data sets:
Data typeCount
13C chemical shifts397
15N chemical shifts98
1H chemical shifts628
coupling constants57
heteronuclear NOE values77
order parameters77
residual dipolar couplings50
T1 relaxation values78
T2 relaxation values78

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1OST_(On-SighT)1

Entities:

Entity 1, OST_(On-SighT) 89 residues - 9979.494 Da.

residues 34-121 from murine GABPa plus C-terminal Trp

1   METALAGLUCYSVALSERGLNALAILEASP
2   ILEASNGLUPROILEGLYASNLEULYSLYS
3   LEULEUGLUPROARGLEUGLNCYSSERLEU
4   ASPALAHISGLUILECYSLEUGLNASPILE
5   GLNLEUASPPROASPARGSERLEUPHEASP
6   GLNGLYVALLYSTHRASPGLYTHRVALGLN
7   LEUSERVALGLNVALILESERTYRGLNGLY
8   METGLUPROLYSLEUASNILELEUGLUILE
9   VALLYSTHRALAGLUTHRVALGLUTRP

Samples:

13C_15N_GABPa_OST: sodium phosphate 20 mM; sodium chloride 20 mM; GABPa OST, [U-100% 13C; U-100% 15N], 1 mM; DTT 2 mM

15N_GABPa_OST: sodium phosphate 20 mM; sodium chloride 50 mM; GABPa OST, [U-100% 15N], 1 mM; DTT 2 mM

15N_10%_13C_GABPa_OST: sodium phosphate 20 mM; sodium chloride 50 mM; GABPa OST, [U-10% 13C; U-100% 15N], 1 mM; DTT 2 mM

sample_conditions: pH: 7.0; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N_GABPa_OSTisotropicsample_conditions
2D 1H-13C HSQC15N_10%_13C_GABPa_OSTisotropicsample_conditions
3D CBCA(CO)NH13C_15N_GABPa_OSTisotropicsample_conditions
3D HNCO13C_15N_GABPa_OSTisotropicsample_conditions
3D HNCA13C_15N_GABPa_OSTisotropicsample_conditions
3D HNCACB13C_15N_GABPa_OSTisotropicsample_conditions
3D 1H-15N NOESY15N_GABPa_OSTisotropicsample_conditions
3D 1H-15N TOCSY13C_15N_GABPa_OSTisotropicsample_conditions
3D HCCH-TOCSY13C_15N_GABPa_OSTisotropicsample_conditions
simultaneous CN NOESY13C_15N_GABPa_OSTisotropicsample_conditions
EZ-HMQC-NH213C_15N_GABPa_OSTisotropicsample_conditions
N-CG spin echo13C_15N_GABPa_OSTisotropicsample_conditions
CO-CG spin echo13C_15N_GABPa_OSTisotropicsample_conditions
15N IPAP15N_GABPa_OSTanisotropicsample_conditions
3D HNHB15N_GABPa_OSTisotropicsample_conditions
T1 T2 HNNOE15N_GABPa_OSTisotropicsample_conditions
3D HNHA15N_GABPa_OSTisotropicsample_conditions

Software:

ARIA v1.2, Brunger, Adams, Clore, Gros, Nilges and Read, Linge, O, . - chemical shift assignment, refinement, structure solution

SPARKY v3.11, Goddard - chemical shift assignment, collection, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

Molmol, Koradi, Billeter and Wuthrich - data analysis, geometry optimization, structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Varian Unity 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAE24181 BAE26442 BAE26687 BAE28337 BAE29575
GB AAA53030 AAH52448 AAO27832 EDK98317 EDM10625
REF NP_001102311 NP_032091 XP_001363875 XP_002716818 XP_003467234
SP Q00422