BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15441

Title: Backbone chemical shift assignments of dynein intermediate chain IC74 residues 84-143   PubMed: 17919953

Authors: Benison, Gregory; Barbar, Elisar; Berkholz, Donald

Citation: Benison, Gregory; Berkholz, Donald; Barbar, Elisar. "Protein Assignments without Peak Lists Using Higher-Order Spectra"  J. Magn. Reson. 189, 173-181 (2007).

Assembly members:
IC74, polymer, 75 residues, Formula weight is not available

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
IC74: SHHHHHHSMANSAGSSNTQT PDASLQATVNGQSGGKKQPL NLSVYNVQATNIPPKETLVY TKQTQTTSTGGGNGD

Data sets:
Data typeCount
13C chemical shifts111
15N chemical shifts55
1H chemical shifts55
heteronuclear NOE values55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IC741

Entities:

Entity 1, IC74 75 residues - Formula weight is not available

1   SERHISHISHISHISHISHISSERMETALA
2   ASNSERALAGLYSERSERASNTHRGLNTHR
3   PROASPALASERLEUGLNALATHRVALASN
4   GLYGLNSERGLYGLYLYSLYSGLNPROLEU
5   ASNLEUSERVALTYRASNVALGLNALATHR
6   ASNILEPROPROLYSGLUTHRLEUVALTYR
7   THRLYSGLNTHRGLNTHRTHRSERTHRGLY
8   GLYGLYASNGLYASP

Samples:

sample_1: IC74, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%; potassium phosphate 50 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 0.25 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-15N hetnoesample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

Burrow-Owl, Benison, Berkholz, and Barbar - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

GB AAC70933 AAC70934 AAC70935 AAC70938 AAC70939
REF NP_001285486 NP_001285487 NP_477070 NP_477072 NP_477074
SP Q24246