BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15438

Title: Solution structure of IPI*   PubMed: 18037438

Authors: Rifat, Dalin; Wright, Nathan; Varney, Kristen; Weber, David; Black, Lindsay

Citation: Rifat, Dalin; Wright, Nathan; Varney, Kristen; Weber, David; Black, Lindsay. "Restriction endonuclease inhibitor IPI* of bacteriophage T4: a novel structure for a dedicated target"  J. Mol. Biol. 375, 720-734 (2008).

Assembly members:
IP1*, polymer, 76 residues, 8060.466 Da.

Natural source:   Common Name: T4 phage   Taxonomy ID: 10665   Superkingdom: Viruses   Kingdom: not available   Genus/species: T4-like viruses Enterobacteria phage T4 sensu lato

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
IP1*: ATLTSEVIKANKGREGKPMI SLVDGEEIKGTVYLGDGWSA KKDGATIVISPAEETALFKA KHISAAHLKIIAKNLL

Data sets:
Data typeCount
13C chemical shifts217
15N chemical shifts76
1H chemical shifts465

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IP1*1

Entities:

Entity 1, IP1* 76 residues - 8060.466 Da.

1   ALATHRLEUTHRSERGLUVALILELYSALA
2   ASNLYSGLYARGGLUGLYLYSPROMETILE
3   SERLEUVALASPGLYGLUGLUILELYSGLY
4   THRVALTYRLEUGLYASPGLYTRPSERALA
5   LYSLYSASPGLYALATHRILEVALILESER
6   PROALAGLUGLUTHRALALEUPHELYSALA
7   LYSHISILESERALAALAHISLEULYSILE
8   ILEALALYSASNLEULEU

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 500 mM; sodium acetate 20 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 20 mM; pH: 4.8; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
4D 15N-13C NOESYsample_1isotropicsample_conditions_1
4D 13C-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CAA26803 CAA84616 CUL01733 CUL02651 CUL03299
GB AAD42585 ACP30784 ADJ39857 AFO10664 AHK10989
REF NP_049749 YP_002854476 YP_009098525 YP_009102346 YP_009102613
SP P03718