BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15431

Title: NMR assignment of the periplasmic oxidoreductase DsbH from Chlamydia   PubMed: 19636863

Authors: Ulmer, Tobias

Citation: Hung, Kuo-Chan; Mac, Thien-Thi; Ulmer, Tobias. "NMR assignment of the periplasmic oxidoreductase DsbH from Chlamydia"  Biomol. NMR Assignments 1, 195-196 (2007).

Assembly members:
DsbH, polymer, 144 residues, 16487.906 Da.

Natural source:   Common Name: Chlamydia pneumoniae   Taxonomy ID: 83558   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Chlamydia pneumoniae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DsbH: ARRRASGENLQQTRPIAAAN LQWESYAEALEHSKQDHKPI GLFFTGSDWCMWCIKMQDQI LQSSEFKHFAGVHLHMVEVD FPQKNHQPEEQRQKNQELKA QYKVTGFPELVFIDAEGKQL ARMGFEPGGGAAYVSKVKSA LKLR

Data sets:
Data typeCount
13C chemical shifts582
15N chemical shifts141
1H chemical shifts880

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1polypeptide chain1

Entities:

Entity 1, polypeptide chain 144 residues - 16487.906 Da.

1   ALAARGARGARGALASERGLYGLUASNLEU
2   GLNGLNTHRARGPROILEALAALAALAASN
3   LEUGLNTRPGLUSERTYRALAGLUALALEU
4   GLUHISSERLYSGLNASPHISLYSPROILE
5   GLYLEUPHEPHETHRGLYSERASPTRPCYS
6   METTRPCYSILELYSMETGLNASPGLNILE
7   LEUGLNSERSERGLUPHELYSHISPHEALA
8   GLYVALHISLEUHISMETVALGLUVALASP
9   PHEPROGLNLYSASNHISGLNPROGLUGLU
10   GLNARGGLNLYSASNGLNGLULEULYSALA
11   GLNTYRLYSVALTHRGLYPHEPROGLULEU
12   VALPHEILEASPALAGLUGLYLYSGLNLEU
13   ALAARGMETGLYPHEGLUPROGLYGLYGLY
14   ALAALATYRVALSERLYSVALLYSSERALA
15   LEULYSLEUARG

Samples:

sample_1: DsbH, [U-99% 13C; U-99% 15N], 1.0 mM; NaH2PO4/Na2HPO4 50 mM; NaN3 0.02 % w/v; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.0; pressure: 1 atm; temperature: 298.2 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1anisotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAA99134
EMBL CRI33456 CRI36320 CRI37446 CRI38577 CRI39708
GB AAD19064 AAF38723 AAP98887 ACZ32813 ETR79697
REF NP_225121 WP_010883561
SP Q9Z6Y0