BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15405

Title: The NMR Solution Structure of Recombinant RGD-hirudin   PubMed: 17585879

Authors: Song, Xia; Mo, Wei; Liu, Xingang; Yan, Xiaomin; Song, Houyan; Dai, Linsen

Citation: Song, Xia; Mo, Wei; Zhu, Lina; Liu, Xingang; Yan, Xiaomin; Song, Houyan; Dai, Linsen. "The NMR Solution Structure of Recombinant RGD-hirudin"  Biochem. Biophys. Res. Commun. 360, 103-108 (2007).

Assembly members:
RGD-hirudin, polymer, 66 residues, 5323.929 Da.

Natural source:   Common Name: Hirudo medicinalis   Taxonomy ID: 6421   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hirudo medicinalis

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris

Entity Sequences (FASTA):
RGD-hirudin: VVYTDCTESGQNLCLCEGSN VCGQGNKCILGRGDSKNQCV TGEGTPKPQSHNQGDFEPIP EDAYDE

Data sets:
Data typeCount
1H chemical shifts410

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RGD-hirudin1

Entities:

Entity 1, RGD-hirudin 66 residues - 5323.929 Da.

1   VALVALTYRTHRASPCYSTHRGLUSERGLY
2   GLNASNLEUCYSLEUCYSGLUGLYSERASN
3   VALCYSGLYGLNGLYASNLYSCYSILELEU
4   GLYARGGLYASPSERLYSASNGLNCYSVAL
5   THRGLYGLUGLYTHRPROLYSPROGLNSER
6   HISASNGLNGLYASPPHEGLUPROILEPRO
7   GLUASPALATYRASPGLU

Samples:

sample_1: RGD-hirudin 5 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

ARIA v2.0, Linge, O, . - refinement

SPARKY v3.105, Goddard - peak picking

Molmol v2000, Koradi, Billeter and Wuthrich - data analysis

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB