BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15392

Title: Tetrameric structure of KIA7F peptide   PubMed: 17855563

Authors: lopez de la Osa, Jaime; Gonzalez, Carlos; Laurents, Douglas; Chakrabartty, Avijit; Bateman, David

Citation: Lopez de la Osa, Jaime; Bateman, David; Ho, S.; Gonzalez, Carlos; Chakrabartty, Avijit; Laurents, Douglas. "Getting specificity from simplicity in putative proteins from the prebiotic earth."  Proc. Natl. Acad. Sci. U.S.A. 104, 14941-14946 (2007).

Assembly members:
KIA7F, polymer, 22 residues, 1853.298 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
KIA7F: XAKAAAAAIKAIAAIIKAGG FX

Data sets:
Data typeCount
1H chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KIA7F peptide, chain 11
2KIA7F peptide, chain 21
3KIA7F peptide, chain 31
4KIA7F peptide, chain 41

Entities:

Entity 1, KIA7F peptide, chain 1 22 residues - 1853.298 Da.

1   ACEALALYSALAALAALAALAALAILELYS
2   ALAILEALAALAILEILELYSALAGLYGLY
3   PHENH2

Samples:

KIA7F_sample: sodium acetate 10 mM; sodium chloride 200 mM; DSS 0.005 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 278.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYKIA7F_sampleisotropicsample_conditions_1
2D 1H-1H TOCSYKIA7F_sampleisotropicsample_conditions_1
2D DQF-COSYKIA7F_sampleisotropicsample_conditions_1

Software:

DYANA v1.5, Guntert, Braun and Wuthrich - structure solution

AMBER v7.0, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Koll - refinement

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker Avance 800 MHz

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