BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15382

Title: Nuclear Magnetic Resonance Studies on Huwentoxin-XI from the Chinese Bird Spider Ornithoctonus huwena   PubMed: 16820861

Authors: Peng, Kuan; Liang, Songping

Citation: Peng, Kuan; Lin, Y.; Liang, Songping. "Nuclear magnetic resonance studies on huwentoxin-XI from the Chinese bird spider Ornithoctonus huwena: 15N labeling and sequence-specific 1H, 15N nuclear magnetic resonance assignments"  Acta Biochim. Biophys. Sin. (Shanghai) 38, 457-466 (2006).

Assembly members:
Huwentoxin-xi, polymer, 55 residues, 6185.185 Da.

Natural source:   Common Name: Chinese bird spider   Taxonomy ID: 29017   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ornithoctonus huwena

Experimental source:   Production method: recombinant technology   Host organism: Saccharomyces cerevisiae strain S-78

Entity Sequences (FASTA):
Huwentoxin-xi: IDTCRLPSDRGRCKASFERW YFNGRTCAKFIYGGCGGNGN KFPTQDACMKRCAKA

Data sets:
Data typeCount
1H chemical shifts374

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Huwentoxin-xi1

Entities:

Entity 1, Huwentoxin-xi 55 residues - 6185.185 Da.

1   ILEASPTHRCYSARGLEUPROSERASPARG
2   GLYARGCYSLYSALASERPHEGLUARGTRP
3   TYRPHEASNGLYARGTHRCYSALALYSPHE
4   ILETYRGLYGLYCYSGLYGLYASNGLYASN
5   LYSPHEPROTHRGLNASPALACYSMETLYS
6   ARGCYSALALYSALA

Samples:

sample_1: Huwentoxin-xi 5 mM

sample_2: Huwentoxin-xi, [U-100% 15N], 5 mM

sample_conditions_1: ionic strength: 50 mM; pH: 4.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB ABY77743 ACD01237 AHY30307 AHY30328 AHY30329
SP P0CH75 P0DJ77 P68425