BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15367

Title: RgIA, a Novel Conotoxin that Blocks the nAChR   PubMed: 18295795

Authors: Feng, Zhi-Ping

Citation: Ellison, Michael; Feng, Zhi-Ping; Park, Anthony; Zhang, Xuecheng; Olivera, Baldomero; McIntosh, Michael; Norton, Raymond. "alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR: Structure and Identification of Key Receptor-Binding Residues"  J. Mol. Biol. 377, 1216-1227 (2008).

Assembly members:
Conotoxin, polymer, 13 residues, 1593.894 Da.

Natural source:   Common Name: Conus regius   Taxonomy ID: 35631   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus regius

Experimental source:   Production method: chemical synthesis   Host organism: snail

Entity Sequences (FASTA):
Conotoxin: GCCSEPRCRYRCR

Data sets:
Data typeCount
1H chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Conotoxin1

Entities:

Entity 1, Conotoxin 13 residues - 1593.894 Da.

1   GLYCYSCYSSERGLUPROARGCYSARGTYR
2   ARGCYSARG

Samples:

sample_1: toxin 1.0 ± 0.2 mM

sample_conditions_1: pH: 4.22; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links: