BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15362

Title: NMR SOLUTION STRUCTURE OF THE ANTICODON OF E.coli TRNA-VAL3 WITH 1 MODIFICATION (M6A37)   PubMed: 18473483

Authors: Vendeix, Franck; Dziergowska, Agnieszka; Gustilo, Estella; Graham, William; Sproat, Brian; Malkiewicz, Andrzej; Agris, Paul

Citation: Vendeix, Franck; Dziergowska, Agnieszka; Gustilo, Estella; Graham, William; Sproat, Brian; Malkiewicz, Andrzej; Agris, Paul. "Anticodon domain modifications contribute order to tRNA for ribosome-mediated codon binding"  Biochemistry 47, 6117-6129 (2008).

Assembly members:
RNA_CCUCCCUUAC6MZAGGAGG, polymer, 17 residues, 5402.314 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
RNA_CCUCCCUUAC6MZAGGAGG: CCUCCCUUACXAGGAGG

Data sets:
Data typeCount
1H chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASLval1

Entities:

Entity 1, ASLval 17 residues - 5402.314 Da.

1   CCUCCCUUAC
2   6MZAGGAGG

Samples:

(M6A37)-ASL_Val3_H2O: RNA 17mer1.5 – 2 mM

(M6A37)-ASL_Val3_D2O: RNA 17mer1.5 – 2 mM

sample_conditions_1: pH: 6.2; pressure: 1 atm; temperature: 295 K

sample_conditions_2: pH: 6.2; pressure: 1 atm; temperature: 275 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSY(M6A37)-ASL_Val3_D2Oisotropicsample_conditions_1
2D DQF-COSY(M6A37)-ASL_Val3_D2Oisotropicsample_conditions_1
2D 1H-1H TOCSY(M6A37)-ASL_Val3_D2Oisotropicsample_conditions_1
2D 1H-13C HSQC(M6A37)-ASL_Val3_D2Oisotropicsample_conditions_1
2D 1H-1H NOESY(M6A37)-ASL_Val3_D2Oisotropicsample_conditions_1
2D 1H-1H NOESY(M6A37)-ASL_Val3_H2Oisotropicsample_conditions_2
2D 1H-31P HETCOR(M6A37)-ASL_Val3_D2Oisotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

VNMR, Varian - collection

NMRDraw v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Varian INOVA 600 MHz

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