BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15359

Title: Solution structure of arenicin-1   PubMed: 17935487

Authors: Jakovkin, Igor; Hecht, Oliver; Gelhaus, Christoph; Krasnosdembskaya, Anna; Fedders, Henning; Leippe, Matthias; Groetzinger, Joachim

Citation: Andra, J.; Jakovkin, Igor; Groetzinger, J.; Hecht, Oliver; Krasnosdembskaya, Anna; Goldman, T.; Gutsmann, T.; Leippe, Matthias. "Structure and mode of action of the antimicrobial peptide arenicin"  Biochem. J. 410, 113-122 (2008).

Assembly members:
arenicin monomer, polymer, 21 residues, 2766.365 Da.

Natural source:   Common Name: lugworm   Taxonomy ID: 6344   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Arenicola marina

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
arenicin monomer: RWCVYAYVRVRGVLVRYRRC W

Data sets:
Data typeCount
1H chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1arenicin monomer1

Entities:

Entity 1, arenicin monomer 21 residues - 2766.365 Da.

1   ARGTRPCYSVALTYRALATYRVALARGVAL
2   ARGGLYVALLEUVALARGTYRARGARGCYS
3   TRP

Samples:

sample_1: entity 1.8 mg; acetic acid, [U-2H], 2%

sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAV65142
SP Q5SC60