BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15345

Title: Solution structure of Blo t 5   PubMed: 18684949

Authors: Chan, Siew Leong; Mok, Yu Keung

Citation: Chan, Siew Leong; Ong, Tan Ching; Gao, Yun Feng; Tiong, Yuen Sung; Wang, De Yun; Chew, Fook Tim; Mok, Yu Keung. "Nuclear Magnetic Resonance Structure and IgE Epitopes of Blo t 5, a Major Dust Mite Allergen"  J. Immunol. 181, 2586-2596 (2008).

Assembly members:
Blo_t_5, polymer, 112 residues, Formula weight is not available

Natural source:   Common Name: Blomia tropicalis   Taxonomy ID: 40697   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Blomia tropicalis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Blo_t_5: KKDDFRNEFDHLLIEQANHA IEKGEHQLLYLQHQLDELNE NKSKELQEKIIRELDVVCAM IEGAQGALERELKRTDLNIL ERFNYEEAQTLSKILLKDLK ETEQKVKDIQTQ

Data sets:
Data typeCount
13C chemical shifts346
15N chemical shifts106
1H chemical shifts518

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Blo t 51

Entities:

Entity 1, Blo t 5 112 residues - Formula weight is not available

1   LYSLYSASPASPPHEARGASNGLUPHEASP
2   HISLEULEUILEGLUGLNALAASNHISALA
3   ILEGLULYSGLYGLUHISGLNLEULEUTYR
4   LEUGLNHISGLNLEUASPGLULEUASNGLU
5   ASNLYSSERLYSGLULEUGLNGLULYSILE
6   ILEARGGLULEUASPVALVALCYSALAMET
7   ILEGLUGLYALAGLNGLYALALEUGLUARG
8   GLULEULYSARGTHRASPLEUASNILELEU
9   GLUARGPHEASNTYRGLUGLUALAGLNTHR
10   LEUSERLYSILELEULEULYSASPLEULYS
11   GLUTHRGLUGLNLYSVALLYSASPILEGLN
12   THRGLN

Samples:

C13_N15: Blo t 5, [U-99% 13C; U-99% 15N], 2 mM

C13: Blo t 5, [U-100% 13C], 2 mM

N15: Blo t 5, [U-99% 15N], 2 mM

sample_conditions_1: ionic strength: 0 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBC13_N15isotropicsample_conditions_1
3D CBCA(CO)NHC13_N15isotropicsample_conditions_1
3D 1H-13C NOESYC13isotropicsample_conditions_1
3D 1H-15N NOESYN15isotropicsample_conditions_1
2D 1H-15N HSQCN15isotropicsample_conditions_1
3D C(CO)NHC13_N15isotropicsample_conditions_1
3D H(CCO)NHC13_N15isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAD10850 ABH06351 ABH06352 ABH06353 ABH06354
SP O96870