BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15343

Title: Solution NMR Structure of CAPER RRM2 Domain. Northeast Structural Genomics Target HR4730A.

Authors: Rossi, Paolo; Zhao, Li; Nwosu, Chioma; Cunningham, Kellie; Owens, Leah; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Swapna, G.V.T.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano

Citation: Rossi, Paolo; Xiao, Rong; Acton, Thomas; Montelione, Gaetano. "Solution NMR Structure of CAPER RRM2 Domain. Northeast Structural Genomics Target HR4730A."  Not known ., .-..

Assembly members:
HR4730A, polymer, 108 residues, 12041.6 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HR4730A: MGHHHHHHSHMAAAMANNLQ KGSAGPMRLYVGSLHFNITE DMLRGIFEPFGRIESIQLMM DSETGRSKGYGFITFSDSEC AKKALEQLNGFELAGRPMKV GHVTERTD

Data sets:
Data typeCount
13C chemical shifts430
15N chemical shifts104
1H chemical shifts680

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HR4730A1

Entities:

Entity 1, HR4730A 108 residues - 12041.6 Da.

1   METGLYHISHISHISHISHISHISSERHIS
2   METALAALAALAMETALAASNASNLEUGLN
3   LYSGLYSERALAGLYPROMETARGLEUTYR
4   VALGLYSERLEUHISPHEASNILETHRGLU
5   ASPMETLEUARGGLYILEPHEGLUPROPHE
6   GLYARGILEGLUSERILEGLNLEUMETMET
7   ASPSERGLUTHRGLYARGSERLYSGLYTYR
8   GLYPHEILETHRPHESERASPSERGLUCYS
9   ALALYSLYSALALEUGLUGLNLEUASNGLY
10   PHEGLULEUALAGLYARGPROMETLYSVAL
11   GLYHISVALTHRGLUARGTHRASP

Samples:

sample_1: HR4730A, [U-100% 13C; U-100% 15N], 1.3 mM; sodium azide 0.02%; MES 20 mM; sodium chloride 100 mM; Calcium Chloride 5 mM; DTT 10 mM

sample_2: HR4730A, [U-5% 13C; U-100% 15N], 1.1 mM; sodium azide 0.02%; MES 20 mM; sodium chloride 100 mM; Calcium Chloride 5 mM; DTT 10 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC stereospecific methylsample_2isotropicsample_conditions_1
HETnoesample_2isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1

Software:

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

AutoStruct v2.1.1, Huang, Tejero, Powers and Montelione - structure solution

NMRPipe v2.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v2.110, Goddard - data analysis

TOPSPIN v1.3, Bruker Biospin - collection

CNSSOLVE v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

X-PLOR NIH v2.11.2, Schwieters, Kuszewski, Tjandra and Clore - refinement

DYANA, Guntert, Mumenthaler and Wuthrich - structure solution

PSVS v1.3, Bhattacharya and Montelione - validation

PDBStat v5.0, (PDBStat) Tejero - data analysis

Molmol v2K.2, Koradi, Billeter and Wuthrich - visualization

MolProbity, Richardson - validation

ProcheckNMR, Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Th - validation

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAE22477 BAE27657 BAE32977 BAG64839 BAJ21051
EMBL CAD97833 CAE45833 CAE45890 CAH18281 CAH90627
GB AAA16346 AAA16347 AAH30493 AAH82607 AAH86645
REF NP_001013225 NP_001125339 NP_001162566 NP_001164806 NP_001193433
SP Q14498 Q5RC80 Q8VH51
TPG DAA23046