BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15336

Title: 1H, 13C and 15N Backbone Resonance Assignments of the Nonphosphorylated CFTR Regulatory Region   PubMed: 17660831

Authors: Baker, Jennifer; Hudson, Rhea; Kanelis, Voula; Choy, Wing-Yiu; Thibodeau, Patrick; Thomas, Philip; Forman-Kay, Julie

Citation: Baker, Jennifer; Hudson, Rhea; Kanelis, Voula; Choy, Wing-Yiu; Thibodeau, Patrick; Thomas, Philip; Forman-Kay, Julie. "CFTR regulatory region interacts with NBD1 predominantly via multiple transient helices."  Nat. Struct. Mol. Biol. 14, 738-745 (2007).

Assembly members:
CFTR_regulatory_region, polymer, 189 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CFTR_regulatory_region: GAMESAERRNSILTETLHRF SLEGDAPVSWTETKKQSFKQ TGEFGEKRKNSILNPINSIR KFSIVQKTPLQMNGIEEDSD EPLERRLSLVPDSEQGEAIL PRISVISTGPTLQARRRQSV LNLMTHSVNQGQNIHRKTTA STRKVSLAPQANLTELDIYS RRLSQETGLEISEEINEEDL KECLFDDME

Data sets:
Data typeCount
13C chemical shifts532
15N chemical shifts176
1H chemical shifts176

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1regulatory region1

Entities:

Entity 1, regulatory region 189 residues - Formula weight is not available

1   GLYALAMETGLUSERALAGLUARGARGASN
2   SERILELEUTHRGLUTHRLEUHISARGPHE
3   SERLEUGLUGLYASPALAPROVALSERTRP
4   THRGLUTHRLYSLYSGLNSERPHELYSGLN
5   THRGLYGLUPHEGLYGLULYSARGLYSASN
6   SERILELEUASNPROILEASNSERILEARG
7   LYSPHESERILEVALGLNLYSTHRPROLEU
8   GLNMETASNGLYILEGLUGLUASPSERASP
9   GLUPROLEUGLUARGARGLEUSERLEUVAL
10   PROASPSERGLUGLNGLYGLUALAILELEU
11   PROARGILESERVALILESERTHRGLYPRO
12   THRLEUGLNALAARGARGARGGLNSERVAL
13   LEUASNLEUMETTHRHISSERVALASNGLN
14   GLYGLNASNILEHISARGLYSTHRTHRALA
15   SERTHRARGLYSVALSERLEUALAPROGLN
16   ALAASNLEUTHRGLULEUASPILETYRSER
17   ARGARGLEUSERGLNGLUTHRGLYLEUGLU
18   ILESERGLUGLUILEASNGLUGLUASPLEU
19   LYSGLUCYSLEUPHEASPASPMETGLU

Samples:

sample_1: CFTR regulatory region, [U-98% 13C; U-98% 15N], 0.2 mM; potassium chloride 125 mM; potassium phosphate 125 mM; DTT 5 mM; EDTA 5 mM; benzamidine 5 mM; DSS 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.25 M; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRView, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, processing

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

BMRB 15340
EMBL CAE00701 CAE00702 CAE00703 CAE00704
GB AAA35680 AAB46352 AAC13657 AAC14011 AAC14012
REF NP_000483 NP_001028110 NP_001073386 NP_001106085 XP_003808695
SP P13569 Q00553 Q2IBF6 Q2QLE5 Q7JII7