BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15333

Title: Resonance Assignment of the human Pirh2 RING domain; Northeast Structural Genomics Consortium Target HT2B   PubMed: 19043414

Authors: Sheng, Yi; Lemak, Alexander; Laister, Rob; Wu, Bin; Yee, Adelinda; Arrowsmith, Cheryl

Citation: Sheng, Yi; Laister, Rob; Lemak, Alexander; Wu, Bin; Tai, Elizabeth; Duran, Shili; Lukin, Jonathan; Sunnerhagen, Maria; Srisailam, Sampath; Karra, Murthy; Benchimol, Sam; Arrowsmith, Cheryl. "Molecular basis of Pirh2-mediated p53 ubiquitylation"  Nat. Struct. Mol. Biol. 15, 1334-1342 (2008).

Assembly members:
Pirh2_RING_domain, polymer, 52 residues, 6007.025 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Pirh2_RING_domain: ENVSQQNCPICLEDIHTSRV VAHVLPCGHLLHRTCYEEML KEGYRCPLCMHS

Data typeCount
13C chemical shifts215
15N chemical shifts49
1H chemical shifts345

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Pirh2 RING domain1
2ZINC ION, 12
3ZINC ION, 22

Entities:

Entity 1, Pirh2 RING domain 52 residues - 6007.025 Da.

1   GLUASNVALSERGLNGLNASNCYSPROILE
2   CYSLEUGLUASPILEHISTHRSERARGVAL
3   VALALAHISVALLEUPROCYSGLYHISLEU
4   LEUHISARGTHRCYSTYRGLUGLUMETLEU
5   LYSGLUGLYTYRARGCYSPROLEUCYSMET
6   HISSER

Entity 2, ZINC ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_2: Pirh2 RING domain, [U-13C; U-15N], 0.7 mM; sodium phosphate 25 mM; potassium chloride 150 mM; ZnCl2 10 uM; DTT, [U-99% 2H], 2 mM; D2O 10%

sample_1: Pirh2 RING domain, [U-13C; U-15N], 0.7 mM; sodium phosphate 25 mM; potassium chloride 150 mM; ZnCl2 10 uM; DTT, [U-99% 2H], 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C_aromatic NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB31179 BAB31236 BAC37254 BAD92309 BAG52375
GB AAH23138 AAH47393 AAH57143 AAH83739 AAK96896
REF NP_001007619 NP_001248474 NP_001258726 NP_001265465 NP_001265467
SP Q96PM5 Q9CR50