BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15322

Title: 1H, 13C, and 15N Chemical Shift Assignments for Chemotaxis Protein CheW   PubMed: 17631272

Authors: Jin, Changwen; Li, You; Fu, Wenyu

Citation: Li, You; Hu, Yunfei; Fu, Wenyu; Xia, Bin; Jin, Changwen. "Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli"  Biochem. Biophys. Res. Commun. 360, 863-867 (2007).

Assembly members:
chemotaxis_protein_CheW, polymer, 167 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
chemotaxis_protein_CheW: MTGMTNVTKLASEPSGQEFL VFTLGDEEYGIDILKVQEIR GYDQVTRIANTPAFIKGVTN LRGVIVPIVDLRIKFSQVDV DYNDNTVVIVLNLGQRVVGI VVDGVSDVLSLTAEQIRPAP EFAVTLSTEYLTGLGALGDR MLILVNIEKLLNSEEMALLD SAASEVA

Data sets:
Data typeCount
13C chemical shifts669
15N chemical shifts168
1H chemical shifts1146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer1

Entities:

Entity 1, monomer 167 residues - Formula weight is not available

1   METTHRGLYMETTHRASNVALTHRLYSLEU
2   ALASERGLUPROSERGLYGLNGLUPHELEU
3   VALPHETHRLEUGLYASPGLUGLUTYRGLY
4   ILEASPILELEULYSVALGLNGLUILEARG
5   GLYTYRASPGLNVALTHRARGILEALAASN
6   THRPROALAPHEILELYSGLYVALTHRASN
7   LEUARGGLYVALILEVALPROILEVALASP
8   LEUARGILELYSPHESERGLNVALASPVAL
9   ASPTYRASNASPASNTHRVALVALILEVAL
10   LEUASNLEUGLYGLNARGVALVALGLYILE
11   VALVALASPGLYVALSERASPVALLEUSER
12   LEUTHRALAGLUGLNILEARGPROALAPRO
13   GLUPHEALAVALTHRLEUSERTHRGLUTYR
14   LEUTHRGLYLEUGLYALALEUGLYASPARG
15   METLEUILELEUVALASNILEGLULYSLEU
16   LEUASNSERGLUGLUMETALALEULEUASP
17   SERALAALASERGLUVALALA

Samples:

sample_1: chemotaxis protein CheW, [U-95% 13C; U-95% 15N], 1 mM; Tris-HCl 30 mM; NaCl 30 mM

sample_conditions_1: ionic strength: 0.06 M; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAA15703 BAB36020 BAG77646 BAI25978 BAI30941
EMBL CAP76375 CAQ32364 CAQ98827 CAR03246 CAR08346
GB AAA23565 AAC74957 AAG56877 AAN43489 AAN80761
PIR A85802
REF NP_310624 NP_416401 NP_707782 WP_000147297 WP_000147298
SP P0A964 P0A965 P0A966 P0A967