BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15318

Title: Antibacterial Peptide from Eggshell Matrix: Structure and Self-assembly of a - defensin Like Peptide from the Chinese Soft-shelled Turtle Eggshell   PubMed: 18341335

Authors: Vivekanandan, Subramanian; Lakshminarayanan, Rajamani; Jois, Seetharama; Perumalsamy, Ramar; Banerjee, Yajnavalka; Ooi Chi-Jin, Emma; Wah Teo, Kay; Kini, Manjunatha; Valiyaveettil, Suresh

Citation: Lakshminarayanan, Rajamani; Vivekanandan, Subramanian; Samy, Ramar; Banerjee, Yajnavalka; Ooi Chi-Jin, Emma; Wah Teo, Kay; Jois, Seetharama; Kini, R.; Valiyaveettil, Suresh. "Structure, self-assembly, and dual role of a beta-defensin-like peptide from the Chinese soft-shelled turtle eggshell matrix"  J. Am. Chem. Soc. 130, 4660-4668 (2008).

Assembly members:
Pelovaterin, polymer, 42 residues, 4201.883 Da.

Natural source:   Common Name: Pelodiscus sinensis   Taxonomy ID: 13735   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Pelodiscus sinensis

Experimental source:   Production method: purified from the natural source   Host organism: Pelodiscus sinensis

Entity Sequences (FASTA):
Pelovaterin: DDTPSSRCGSGGWGPCLPIV DLLCIVHVTVGCSGGFGCCR IG

Data sets:
Data typeCount
1H chemical shifts250

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Pelovaterin1

Entities:

Entity 1, Pelovaterin 42 residues - 4201.883 Da.

1   ASPASPTHRPROSERSERARGCYSGLYSER
2   GLYGLYTRPGLYPROCYSLEUPROILEVAL
3   ASPLEULEUCYSILEVALHISVALTHRVAL
4   GLYCYSSERGLYGLYPHEGLYCYSCYSARG
5   ILEGLY

Samples:

sample_1: Pelovaterin 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, P.GUNTERT ET AL. - refinement, structure solution

SPARKY v3.110, Goddard - data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 500 MHz

Related Database Links:

PDB
SP P84818