BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15316

Title: Electrostatic contributions to the stability of the GCN4 leucine zipper structure.   PubMed: 17920624

Authors: Matousek, William; Alexandrescu, Andrei

Citation: Matousek, William; Ciani, Barbara; Fitch, Carolyn; Garcia-Moreno, Bertrand; Kammerer, Richard; Alexandrescu, Andrei. "Electrostatic contributions to the stability of the GCN4 leucine zipper structure."  J. Mol. Biol. 374, 206-219 (2007).

Assembly members:
GCN4p-wt, polymer, 33 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
GCN4p-wt: GSMKQLEDKVEELLSKNYHL ENEVARLKKLVGE

Data sets:
Data typeCount
13C chemical shifts142
15N chemical shifts31
1H chemical shifts220

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GCN41

Entities:

Entity 1, GCN4 33 residues - Formula weight is not available

Leucine zipper region of GCN4 yeast transcription activator Met250 Glu280

1   GLYSERMETLYSGLNLEUGLUASPLYSVAL
2   GLUGLULEULEUSERLYSASNTYRHISLEU
3   GLUASNGLUVALALAARGLEULYSLYSLEU
4   VALGLYGLU

Samples:

Native_GCN4p-wt_1: GCN4p-wt, [U-13C; U-15N], 2.1 mM; sodium chloride 150 mM; sodium phosphate 10 mM; D2O 10%

Native_GCN4p-wt_cond: pH: 6.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBNative_GCN4p-wt_1isotropicNative_GCN4p-wt_cond
3D HNCONative_GCN4p-wt_1isotropicNative_GCN4p-wt_cond
3D HCCH-TOCSYNative_GCN4p-wt_1isotropicNative_GCN4p-wt_cond
3D 1H-13C NOESYNative_GCN4p-wt_1isotropicNative_GCN4p-wt_cond

Software:

FELIX v2000.1, Accelrys Software Inc. - chemical shift assignment, peak picking, processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 1396 1397 1398 1399 1451 1452 1453 1454 17511 2062 371
PDB
DBJ GAA22815
EMBL CAE52206 CAE52207 CAE52208 CAE52209 CAE52210
GB AAA34640 AAB64486 AAC33186 AAD37724 AAK07887
REF NP_010907
SP P03069
TPG DAA07643