BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15299

Title: AgTx2-MTX Toxin   PubMed: 18042681

Authors: Pimentel, Cyril; M'Barek, Sarrah; Visan, Violetta; Grissmer, Stephan; Sampieri, Francois; Sabatier, Jean-Marc; Darbon, Herve; Fajloun, Ziad

Citation: Pimentel, Cyril; M'Barek, Sarrah; Visan, Violetta; Grissmer, Stephan; Sampieri, Francois; Sabatier, Jean-Marc; Darbon, Herve; Fajloun, Ziad. "Chemical synthesis and 1H-NMR 3D structure determination of AgTx2-MTX chimera, a new potential blocker for Kv1.2 channel, derived from MTX and AgTx2 scorpion toxins"  Protein Sci. 17, 107-118 (2008).

Assembly members:
AgTx2-MTX, polymer, 39 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
AgTx2-MTX: GVPINVSCTGSKDCYAPCRK QTGCPNAKCINKSCKCYGC

Data sets:
Data typeCount
1H chemical shifts217

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AgTx2-MTX1

Entities:

Entity 1, AgTx2-MTX 39 residues - Formula weight is not available

1   GLYVALPROILEASNVALSERCYSTHRGLY
2   SERLYSASPCYSTYRALAPROCYSARGLYS
3   GLNTHRGLYCYSPROASNALALYSCYSILE
4   ASNLYSSERCYSLYSCYSTYRGLYCYS

Samples:

sample_1: AgTx2-MTX 1 mM

sample_conditions_1: ionic strength: 0 M; pH: 3; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

XEASY v2.1, Bartels et al. - chemical shift assignment, peak picking

ARIA v1.2, Linge, O, . - refinement, structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
SP P80719