BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15288

Title: NMR solution structure of Bacillus subtilis YobA 21-120: Northeast Structural Genomics Consortium target SR547

Authors: Cort, John; Ramelot, Theresa; Cort, Chen; Jang, Mei; Cunningham, Kellie; Ma, Li-Chung; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Kennedy, Michael

Citation: Cort, John. "NMR solution structure of Bacillus subtilis YobA 21-120"  . ., .-..

Assembly members:
YobA 21-120, polymer, 108 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
YobA 21-120: MNKNEQNGDETKMQSLVGYV VLKDNERAILITDTKAPGKE DYNLSEGQLMNKFKNNIVIV GLSEIDNTDDLKRGEKIKVW FHTRKESNPPSATIQKYELL LEHHHHHH

Data sets:
Data typeCount
13C chemical shifts456
15N chemical shifts117
1H chemical shifts751

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YobA 21-1201

Entities:

Entity 1, YobA 21-120 108 residues - Formula weight is not available

Residue 2 in this entry corresponds to residue 21 in the public database sequence; the first 20 are a putative signal peptide sequence and have been truncated.

1   METASNLYSASNGLUGLNASNGLYASPGLU
2   THRLYSMETGLNSERLEUVALGLYTYRVAL
3   VALLEULYSASPASNGLUARGALAILELEU
4   ILETHRASPTHRLYSALAPROGLYLYSGLU
5   ASPTYRASNLEUSERGLUGLYGLNLEUMET
6   ASNLYSPHELYSASNASNILEVALILEVAL
7   GLYLEUSERGLUILEASPASNTHRASPASP
8   LEULYSARGGLYGLULYSILELYSVALTRP
9   PHEHISTHRARGLYSGLUSERASNPROPRO
10   SERALATHRILEGLNLYSTYRGLULEULEU
11   LEUGLUHISHISHISHISHISHIS

Samples:

NC: entity, [U-100% 13C; U-100% 15N], 0.75 mM; dithiothreitol 10 mM; calcium chloride 5 mM; sodium chloride 100 mM; ammonium acetate 20 mM; sodium azide 0.02%; H2O 95%; D2O 5%

NC5: entity, [5% 13C; U-100% N15], 0.79 mM; dithiothreitol 10 mM; calcium chloride 5 mM; sodium chloride 100 mM; ammonium acetate 20 mM; sodium azide 0.02%; H2O 95%; D2O 5%

NC_D2O: entity, [U-100% 13C; U-100% 15N], 0.75 mM; dithiothreitol 10 mM; calcium chloride 5 mM; sodium chloride 100 mM; ammonium acetate 20 mM; sodium azide 0.02%; D2O 100%

sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNCisotropicsample_conditions_1
2D 1H-13C HSQCNC5isotropicsample_conditions_1
3D CBCA(CO)NHNCisotropicsample_conditions_1
3D HNCACBNCisotropicsample_conditions_1
3D HBHA(CO)NHNCisotropicsample_conditions_1
3D HCCH-TOCSYNCisotropicsample_conditions_1
3D HNHANCisotropicsample_conditions_1
3D HNCONCisotropicsample_conditions_1
3D CCH-TOCSYNCisotropicsample_conditions_1
3D 1H-15N NOESYNCisotropicsample_conditions_1
3D 1H-13C NOESYNCisotropicsample_conditions_1
4D 1H-13C-13C-1H HMQC-NOESYNC_D2Oisotropicsample_conditions_1

Software:

AutoStruct, Huang, Tejero, Powers and Montelione - structure solution

SPARKY, Goddard - data analysis

FELIX, Accelrys Software Inc. - processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

PSVS, Bhattacharya and Montelione - structure evaluation

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz

Related Database Links:

PDB
DBJ BAM52538 BAM58113
EMBL CAB13773 CEI57077 CEJ77502
GB ADV92789 AFQ57816 AGG61259 AHA77924 AIC40308
REF NP_389762 WP_003231383 WP_015714070 WP_029318065 WP_038429094
SP O31835