BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15283

Title: 13C and 15N Chemical Shift Assignments and Secondary Structure of the B3 Immunoglobulin-Binding Domain of Streptococcal Protein G (GB3) by Magic-Angle Spinning Solid-State NMR Spectroscopy   PubMed: 19636843

Authors: Nadaud, Philippe; Helmus, Jonathan; Jaroniec, Christopher

Citation: Nadaud, Philippe; Helmus, Jonathan; Jaroniec, Christopher. "13C and 15N chemical shift assignments and secondary structure of the B3 immunoglobulin-binding domain of streptococcal protein G by magic-angle spinning solid-state NMR spectroscopy"  Biomol. NMR Assignments 1, 117-120 (2007).

Assembly members:
GB3, polymer, 56 residues, 6207.8 Da.

Natural source:   Common Name: Streptococcus sp. 'group G'   Taxonomy ID: 1320   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus Streptococcus sp. (group G)

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
GB3: MQYKLVINGKTLKGETTTKA VDAETAEKAFKQYANDNGVD GVWTYDDATKTFTVTE

Data sets:
Data typeCount
13C chemical shifts272
15N chemical shifts69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GB31

Entities:

Entity 1, GB3 56 residues - 6207.8 Da.

1   METGLNTYRLYSLEUVALILEASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRLYSALA
3   VALASPALAGLUTHRALAGLULYSALAPHE
4   LYSGLNTYRALAASNASPASNGLYVALASP
5   GLYVALTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALTHRGLU

Samples:

sample_1: GB3, [U-100% 13C; U-100% 15N], 20 mg; 2-methylpentane-2,4-diol 0.5 v/v; isopropanol 0.25 v/v; H2O 0.25 v/v; sodium phosphate 50 mM; sodium azide 0.02%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 278 K; temperature controller setting: 273 K

Experiments:

NameSampleSample stateSample conditions
2D CC (DARR mixing)sample_1solidsample_conditions_1
2D N(CA)CXsample_1solidsample_conditions_1
2D N(CO)CXsample_1solidsample_conditions_1
3D NCACXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
3D CONCAsample_1solidsample_conditions_1

Software:

VNMRJ v2.1B, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian Varian NMR System 500 MHz

Related Database Links:

BMRB 18531 2575 25807
PDB
GB AAA26921 AAB27024 ABL60854 ABL60859 ABO76907