BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15276

Title: 1H, 13C and 15N resonance assignment of 6aJL2(R25G), a highly fibrillogenic lamdaVI light chain variable domain.   PubMed: 19636854

Authors: Gutierrez-Gonzalez, Luis; Muresanu, Lucia; del Pozo-Yauner, Luis; Sanchez, Rosalba; Guereca, Leopoldo; Becerril, Baltazar; Luecke, Christian

Citation: Gutierrez-Gonzalez, Luis; Muresanu, Lucia; del Pozo-Yauner, Luis; Sanchez, Rosalba; Guereca, Leopoldo; Becerril, Baltazar; Lucke, Christian. "1H, 13C and 15N resonance assignment of 6aJL2(R25G), a highly fibrillogenic lamdaVI light chain variable domain."  Biomol. NMR Assignments 1, 159-161 (2007).

Assembly members:
6aJL2, polymer, 111 residues, 11853.8 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
6aJL2: NFMLTQPHSVSESPGKTVTI SCTGSSGSIASNYVQWYQQR PGSSPTTVIYEDNQRPSGVP DRFSGSIDSSSNSASLTISG LKTEDEADYYCQSYDSSNHV VFGGGTKLTVL

Data sets:
Data typeCount
13C chemical shifts464
15N chemical shifts119
1H chemical shifts713

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
16aJL21

Entities:

Entity 1, 6aJL2 111 residues - 11853.8 Da.

Residue number 20 does not exist in this sequence numbering

1   ASNPHEMETLEUTHRGLNPROHISSERVAL
2   SERGLUSERPROGLYLYSTHRVALTHRILE
3   SERCYSTHRGLYSERSERGLYSERILEALA
4   SERASNTYRVALGLNTRPTYRGLNGLNARG
5   PROGLYSERSERPROTHRTHRVALILETYR
6   GLUASPASNGLNARGPROSERGLYVALPRO
7   ASPARGPHESERGLYSERILEASPSERSER
8   SERASNSERALASERLEUTHRILESERGLY
9   LEULYSTHRGLUASPGLUALAASPTYRTYR
10   CYSGLNSERTYRASPSERSERASNHISVAL
11   VALPHEGLYGLYGLYTHRLYSLEUTHRVAL
12   LEU

Samples:

sample_1: 6aJL2 1.2 mM; sodium phosphate 25 mM; sodium azide 0.05%; H2O 95%; D2O 5%

sample_2: 6aJL2, [U-95% 15N], 1.2 mM; sodium phosphate 25 mM; sodium azide 0.05%; H2O 95%; D2O 5%

sample_3: 6aJL2, [U-95% 13C; U-95% 15N], 1.2 mM; sodium phosphate 25 mM; sodium azide 0.05%; H2O 95%; D2O 5%

sample_4: 6aJL2 1.3 mM; sodium phosphate 25 mM; sodium azide 0.05%; D2O 100%

sample_conditions_1: ionic strength: 25 mM; pH: 5.2; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HTQCsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HNCACOsample_3isotropicsample_conditions_1
3D C(CO)NHsample_3isotropicsample_conditions_1
3D H(CCO)NHsample_3isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_3isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
2D 1H-1H TOCSYsample_4isotropicsample_conditions_1
2D 1H-1H NOESYsample_4isotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection

AURELIA v3.1.6, Neidig, Geyer, Gorler, Antz, Saffrich, Beneicke, Kalbitzer - data analysis

FELIX v2000, Accelrys Software Inc. - data analysis

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 19798 19870
PDB
DBJ BAA19991 BAC01822 BAC01823 BAC01824 BAC01857
EMBL CAD43014 CAJ75494 CAJ75495 CAP74492 CAP74493
GB AAB33217 AAG24687 AAG24691 AAG47681 AAM46210