BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15274

Title: NMR chemical shift assignments for Arf-binding domain of Hdm2   PubMed: 18809412

Authors: Sivakolundu, Sivashankar; Kriwacki, Richard

Citation: Sivakolundu, Sivashankar; Nourse, Amanda; Moshiach, Simon; Bothner, Brian; Ashley, Chimere; Satumba, John; Lahti, Jill; Kriwacki, Richard. "Intrinsically Unstructured Domains of Arf and Hdm2 Form Bimolecular Oligomeric Structures In Vitro and In Vivo"  J. Mol. Biol. 384, 240-254 (2008).

Assembly members:
Hdm2-ABD, polymer, 99 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Hdm2-ABD: GSHMSSSSESTGTPSNPDLD AGVSEHSGDWLDQDSVSDQF SVEFEVESLDSEDYSLSEEG QELSDEDDEVYQVTVYQAGE SDTDSFEEDPEISLADYWK

Data sets:
Data typeCount
13C chemical shifts276
15N chemical shifts91
1H chemical shifts91

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hdm2-ABD1

Entities:

Entity 1, Hdm2-ABD 99 residues - Formula weight is not available

1   GLYSERHISMETSERSERSERSERGLUSER
2   THRGLYTHRPROSERASNPROASPLEUASP
3   ALAGLYVALSERGLUHISSERGLYASPTRP
4   LEUASPGLNASPSERVALSERASPGLNPHE
5   SERVALGLUPHEGLUVALGLUSERLEUASP
6   SERGLUASPTYRSERLEUSERGLUGLUGLY
7   GLNGLULEUSERASPGLUASPASPGLUVAL
8   TYRGLNVALTHRVALTYRGLNALAGLYGLU
9   SERASPTHRASPSERPHEGLUGLUASPPRO
10   GLUILESERLEUALAASPTYRTRPLYS

Samples:

sample_1: Hdm2-ABD, [U-98% 13C; U-98% 15N], 1 mM; sodium phosphate 10 mM; NaCl 10 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.02 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

FELIX, Accelrys Software Inc. - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz
  • Varian INOVA 600 MHz

Related Database Links:

DBJ BAB11975 BAC78209 BAF83030 BAJ17752
EMBL CAA78055 CAH89564 CAP16704 CAP16705 CAP16729
GB AAA60568 AAA75514 AAA75516 AAF28866 AAF42995
PRF 1814460A
REF NP_001009346 NP_001092577 NP_001124685 NP_001138811 NP_001138812
SP P56950 P56951 Q00987 Q7YRZ8
TPG DAA29805