BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15270

Title: Solution structure of RPA3114, a SEC-C motif containing protein from Rhodopseudomonas palustris; Northeast Structural Genomics Consortium target RpT5 / Ontario Center for Structural Proteomics target RP3097

Authors: Lukin, Jonathan; Gutmanas, Aleksandras; Karra, Murthy; Semesi, Anthony

Citation: Lemak, Alexander; Lukin, Jonathan; Yee, Adelinda; Gutmanas, Aleksandras; Karra, Murthy; Semesi, Anthony; Arrowsmith, Cheryl. "Solution structure of a Se-C motif containing protein from Rhodopseudomonas palustris"  Not known ., .-..

Assembly members:
RP3097, polymer, 128 residues, 14665.401 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: not available   Taxonomy ID: 1076   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Rhodopseudomonas palustris

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RP3097: MNCVCGSGKTYDDCCGPLLA RTRSAASPEALMRSRYAAYA LKDFDYIVETTDPERRDLFD HDVNRAWMEESDFLELRVLG SSEKGSRGTVEFIARFRRGG GPEQSHHERSQFRKARGRWY FSEGEAVD

Data sets:
Data typeCount
13C chemical shifts515
15N chemical shifts121
1H chemical shifts798

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RP30971
2ZINC ion2

Entities:

Entity 1, RP3097 128 residues - 14665.401 Da.

1   METASNCYSVALCYSGLYSERGLYLYSTHR
2   TYRASPASPCYSCYSGLYPROLEULEUALA
3   ARGTHRARGSERALAALASERPROGLUALA
4   LEUMETARGSERARGTYRALAALATYRALA
5   LEULYSASPPHEASPTYRILEVALGLUTHR
6   THRASPPROGLUARGARGASPLEUPHEASP
7   HISASPVALASNARGALATRPMETGLUGLU
8   SERASPPHELEUGLULEUARGVALLEUGLY
9   SERSERGLULYSGLYSERARGGLYTHRVAL
10   GLUPHEILEALAARGPHEARGARGGLYGLY
11   GLYPROGLUGLNSERHISHISGLUARGSER
12   GLNPHEARGLYSALAARGGLYARGTRPTYR
13   PHESERGLUGLYGLUALAVALASP

Entity 2, ZINC ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: entity_1, [U-13C; U-15N], 0.5 mM; TRIS, [U-99% 2H], 10 mM; DTT, [U-99% 2H], 10 mM; sodium chloride 300 mM; sodium azide 0.1%; benzamidine 1 mM; ZnS04 10 uM; H2O 95%; D2O 5%

sample_2: entity_1, [U-13C; U-15N], 0.5 mM; TRIS, [U-99% 2H], 10 mM; DTT, [U-99% 2H], 10 mM; sodium chloride 300 mM; sodium azide 0.1%; benzamidine 1 mM; ZnS04 10 uM; D2O 100%

sample_conditions_1: ionic strength: 300 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY_aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY_aromaticsample_2isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY, Goddard - peak picking

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CAE28555
GB ACF02024
REF WP_011158659