BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15255

Title: DtxR SH3   PubMed: 17976643

Authors: Bhattacharya, Nilakshee

Citation: Bhattacharya, Nilakshee; Yi, M.; Zhou, H.; Logan, T.. "Backbone dynamics in an intramolecular prolylpeptide-SH3 complex from the diphtheria toxin repressor, DtxR"  J. Mol. Biol. 374, 977-992 (2007).

Assembly members:
SH3, polymer, 87 residues, Formula weight is not available

Natural source:   Common Name: Corynebacterium diphtheriae   Taxonomy ID: 1717   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Corynebacterium diphtheriae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SH3: GSHMDAAAPGTRVIDAATSM PRKVRIVQINEIFQVETDQF TQLLDADIRVGSEVEIVDRD GHITLSHNGKDVELLDDLAH TIRIEEL

Data sets:
Data typeCount
heteronuclear NOE values73
T1 relaxation values150
T2 relaxation values149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 87 residues - Formula weight is not available

1   GLYSERHISMETASPALAALAALAPROGLY
2   THRARGVALILEASPALAALATHRSERMET
3   PROARGLYSVALARGILEVALGLNILEASN
4   GLUILEPHEGLNVALGLUTHRASPGLNPHE
5   THRGLNLEULEUASPALAASPILEARGVAL
6   GLYSERGLUVALGLUILEVALASPARGASP
7   GLYHISILETHRLEUSERHISASNGLYLYS
8   ASPVALGLULEULEUASPASPLEUALAHIS
9   THRILEARGILEGLUGLULEU

Samples:

sample_1: SH3, [U-15N], 0.8 mM; K Phos 10 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
15N T1sample_1isotropicsample_conditions_1
15N T1sample_1isotropicsample_conditions_1
15N T2sample_1isotropicsample_conditions_1
15N T2sample_1isotropicsample_conditions_1
Het NOEsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 720 MHz

Related Database Links:

BMRB 15254 4183
PDB
EMBL CAE49945 CCG27916 CCG27917 CCG27918 CCG27919
GB AAA23296 AAA23299 AAA23301 AAU93781 AAU93782
REF WP_003851803 WP_010935052 WP_014316797 WP_014320173
SP H2I233 P0DJL7