BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15250

Title: Main chain NMR assignments of SBT70   PubMed: 19636867

Authors: Sari, Nese; Bryan, Philip; Orban, John

Citation: Sari, Nese; Fisher, Kathryn; Bryan, Philip; Orban, John. "Main chain NMR assignments of subtilisin Sbt70 in its prodomain-bound state"  Biomol. NMR Assignments 1, 209-211 (2007).

Assembly members:
Sbt70, polymer, 266 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Sbt70: AQSVPYGVSQIKAPALHSQG YTGSNVKVAVIDSGIDSSHP DLNVAGGASFVPSETNPFQD NNSHGTHVAGTVLAVAPSAS LYAVKVLGADGSGQYSWIIN GIEWAIANNMDVINMSLGGP SGSAALKAAVDKAVASGVVV VAAAGNEGTSGSSSTVGYPG KYPSVIAVGAVDSSNQRASF SSVGPELDVMAPGVSIVSTL PGNKYGAKSGTAMASPHVAG AAALILSKHPNWTNTQVRSS LENTTTKLGDSFYYGKGLIN VEAAAQ

Data sets:
Data typeCount
13C chemical shifts594
15N chemical shifts193
1H chemical shifts193

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sbt701

Entities:

Entity 1, Sbt70 266 residues - Formula weight is not available

1   ALAGLNSERVALPROTYRGLYVALSERGLN
2   ILELYSALAPROALALEUHISSERGLNGLY
3   TYRTHRGLYSERASNVALLYSVALALAVAL
4   ILEASPSERGLYILEASPSERSERHISPRO
5   ASPLEUASNVALALAGLYGLYALASERPHE
6   VALPROSERGLUTHRASNPROPHEGLNASP
7   ASNASNSERHISGLYTHRHISVALALAGLY
8   THRVALLEUALAVALALAPROSERALASER
9   LEUTYRALAVALLYSVALLEUGLYALAASP
10   GLYSERGLYGLNTYRSERTRPILEILEASN
11   GLYILEGLUTRPALAILEALAASNASNMET
12   ASPVALILEASNMETSERLEUGLYGLYPRO
13   SERGLYSERALAALALEULYSALAALAVAL
14   ASPLYSALAVALALASERGLYVALVALVAL
15   VALALAALAALAGLYASNGLUGLYTHRSER
16   GLYSERSERSERTHRVALGLYTYRPROGLY
17   LYSTYRPROSERVALILEALAVALGLYALA
18   VALASPSERSERASNGLNARGALASERPHE
19   SERSERVALGLYPROGLULEUASPVALMET
20   ALAPROGLYVALSERILEVALSERTHRLEU
21   PROGLYASNLYSTYRGLYALALYSSERGLY
22   THRALAMETALASERPROHISVALALAGLY
23   ALAALAALALEUILELEUSERLYSHISPRO
24   ASNTRPTHRASNTHRGLNVALARGSERSER
25   LEUGLUASNTHRTHRTHRLYSLEUGLYASP
26   SERPHETYRTYRGLYLYSGLYLEUILEASN
27   VALGLUALAALAALAGLN

Samples:

sample_1: Sbt70, [U-95% 13C; U-95% 15N], 0.7 mM; KPi 0.1 M; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.2; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.110, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 15248
PDB