BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15244

Title: 1H, 15N chemical shift backbone assignment of the E19K,D22K mutant of the protein CyaY   PubMed: 19305405

Authors: Pastore, Chiara; Pastore, Annalisa; Correia, Ana; Gomes, Claudio

Citation: Adinolfi, Salvatore; Iannuzzi, Clara; Prischi, Filippo; Pastore, Chiara; Iametti, Stefania; Martin, Stephen; Bonomi, Franco; Pastore, Annalisa. "Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS."  Nat. Struct. Mol. Biol. 16, 390-396 (2009).

Assembly members:
CyaYE19K,D22K, polymer, 108 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CyaYE19K,D22K: GAMNDSEFHRLADQLWLTIE KRLKDWDGDSDIDCEINGGV LTITFENGSKIIINRQEPLH QVWLATKQGGYHFDLKGDEW ICDRSGETFWDLLEQAATQQ AGETVSFR

Data sets:
Data typeCount
15N chemical shifts102
1H chemical shifts102

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CyaYE19K,D22K1

Entities:

Entity 1, CyaYE19K,D22K 108 residues - Formula weight is not available

1   GLYALAMETASNASPSERGLUPHEHISARG
2   LEUALAASPGLNLEUTRPLEUTHRILEGLU
3   LYSARGLEULYSASPTRPASPGLYASPSER
4   ASPILEASPCYSGLUILEASNGLYGLYVAL
5   LEUTHRILETHRPHEGLUASNGLYSERLYS
6   ILEILEILEASNARGGLNGLUPROLEUHIS
7   GLNVALTRPLEUALATHRLYSGLNGLYGLY
8   TYRHISPHEASPLEULYSGLYASPGLUTRP
9   ILECYSASPARGSERGLYGLUTHRPHETRP
10   ASPLEULEUGLUGLNALAALATHRGLNGLN
11   ALAGLYGLUTHRVALSERPHEARG

Samples:

sample_1: CyaYE19K,D22K, [U-98% 15N], 0.7 mM; Tris-HCl 20 mM; NaCl 50 mM

sample_conditions_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15237
PDB
DBJ BAB38160 BAE77494 BAG79612 BAI27941 BAI33064
EMBL CAA47281 CAP78250 CAQ34148 CAQ91159 CAR00776
GB AAA67603 AAC76810 AAG58999 AAN45316 AAP18882
REF NP_312764 NP_418251 NP_709609 WP_000999934 WP_000999936
SP A1AHX3 A7ZU09 A8A6R1 B1IW97 B1LLX7