BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15236

Title: 1H, 15N backbone chemical shift assignemnt of the G26K,D27S,D31A triple mutant of the protein CyaY   PubMed: 18537827

Authors: Pastore, Chiara; Pastore, Annalisa; Correia, Ana; Gomes, Claudio

Citation: Correia, Ana; Pastore, Chiara; Adinolfi, Salvatore; Pastore, Annalisa; Gomes, Claudio. "Dynamics, stability and iron-binding activity of frataxin clinical mutants."  FEBS J. 275, 3680-3690 (2008).

Assembly members:
CyaYG26KD27SD31K, polymer, 108 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CyaYG26KD27SD31K: GAMNDSEFHRLADQLWLTIE ERLDDWDKSSDIACEINGGV LTITFENGSKIIINRQEPLH QVWLATKQGGYHFDLKGDEW ICDRSGETFWDLLEQAATQQ AGETVSFR

Data sets:
Data typeCount
15N chemical shifts104
1H chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CyaYG26KD27SD31K1

Entities:

Entity 1, CyaYG26KD27SD31K 108 residues - Formula weight is not available

1   GLYALAMETASNASPSERGLUPHEHISARG
2   LEUALAASPGLNLEUTRPLEUTHRILEGLU
3   GLUARGLEUASPASPTRPASPLYSSERSER
4   ASPILEALACYSGLUILEASNGLYGLYVAL
5   LEUTHRILETHRPHEGLUASNGLYSERLYS
6   ILEILEILEASNARGGLNGLUPROLEUHIS
7   GLNVALTRPLEUALATHRLYSGLNGLYGLY
8   TYRHISPHEASPLEULYSGLYASPGLUTRP
9   ILECYSASPARGSERGLYGLUTHRPHETRP
10   ASPLEULEUGLUGLNALAALATHRGLNGLN
11   ALAGLYGLUTHRVALSERPHEARG

Samples:

sample_1: CyaYG26KD27SD31K, [U-98% 15N], 0.4 mM; NaP 20 mM; NaCl 50 mM; DTT 2 mM

sample_conditions_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

BMRB 15237
PDB
DBJ BAE77494 BAG79612 BAI27941 BAI33064 BAI38380
EMBL CAA47281 CAR00776 CAR15464 CAV00925 CBJ03590
GB AAA67603 AAC76810 AAZ90503 ABB63872 ABV17629
REF NP_418251 WP_000999947 WP_032267531 WP_042109681 WP_042113898
SP A7ZU09 B1IW97 B1XAH3 B6I4E2 B7L963