BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15229

Title: ATOMIC STRUCTURE OF TRANSLATION INITIATION FACTOR aIF2 beta-SUBUNIT FROM ARCHAEBACTERIA SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION NMR IN SOLUTION   PubMed: 18004773

Authors: Vasile, F.; Pechkova, E.; Stolboushkina, E.; Garber, M.; Nicolini, C.

Citation: Vasile, F.; Pechkova, E.; Nicolini, C.. "Solution structure of the beta-subunit of the translation initiation factor aIF2 from archaebacteria Sulfolobus solfataricus."  Proteins 70, 1112-1115 (2008).

Assembly members:
Translation_initiation_factor_2_beta_subunit, polymer, 146 residues, 16799 Da.

Natural source:   Common Name: Sulfolobus solfataricus   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Translation_initiation_factor_2_beta_subunit: HHHHHHMGSSEKEYVEMLDR LYSKLPEKGRKEGTQSLPNM IILNIGNTTIIRNFAEYCDR IRREDKICMKYLLKELAAPG NVDDKGELVIQGKFSSQVIN TLMERFLKAYVECSTCKSLD TILKKEKKSWYIVCLACGAQ TPVKPL

Data sets:
Data typeCount
1H chemical shifts848

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit beta1

Entities:

Entity 1, subunit beta 146 residues - 16799 Da.

1   HISHISHISHISHISHISMETGLYSERSER
2   GLULYSGLUTYRVALGLUMETLEUASPARG
3   LEUTYRSERLYSLEUPROGLULYSGLYARG
4   LYSGLUGLYTHRGLNSERLEUPROASNMET
5   ILEILELEUASNILEGLYASNTHRTHRILE
6   ILEARGASNPHEALAGLUTYRCYSASPARG
7   ILEARGARGGLUASPLYSILECYSMETLYS
8   TYRLEULEULYSGLULEUALAALAPROGLY
9   ASNVALASPASPLYSGLYGLULEUVALILE
10   GLNGLYLYSPHESERSERGLNVALILEASN
11   THRLEUMETGLUARGPHELEULYSALATYR
12   VALGLUCYSSERTHRCYSLYSSERLEUASP
13   THRILELEULYSLYSGLULYSLYSSERTRP
14   TYRILEVALCYSLEUALACYSGLYALAGLN
15   THRPROVALLYSPROLEU

Samples:

sample: Translation initiation factor 2 beta subunit 0.6 mM; sodium phosphate 50 mM; NaCl 10 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.2 M; pH: 8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYsampleisotropicsample_conditions_1
2D NOESYsampleisotropicsample_conditions_1

Software:

xwinnmr v2.6, Bruker - collection

XEASY v1.3.13, P. Guntert and K. Wuthrich et al - data analysis

DYANA v1.5, P. Guntert and K. Wuthrich et al - structure solution

GROMACS v3.3.1, David van der Spoel et al. - refinement

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

PDB
GB AAK42529 ACX90486 AKA72626 AKA75325 AKA78018
REF WP_009989461
SP Q97W59