BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15222

Title: PV-3C   PubMed: 18305026

Authors: Amero, Carlos; Arnold, Jamie; Cameron, Craig; Foster, Mark

Citation: Amero, Carlos; Arnold, Jamie; Moustafa, I.; Cameron, Craig; Foster, Mark. "Identification of the oriI-binding site of poliovirus 3C protein by nuclear magnetic resonance spectroscopy"  J. Virol. 82, 4363-4370 (2008).

Assembly members:
3C, polymer, 193 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 138953   Superkingdom: Viruses   Kingdom: not available   Genus/species: Enterovirus Poliovirus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
3C: GPGFDYAVAMAKRNIVTATT SKGEFTMLGVHDNVAILPTH ASPGESIVIDGKEVEILDAK ALEDQAGTNLEITIITLKRN EKFRDIRPHIPTQITETNDG VLIVNTSKYPNMYVPVGAVT EQGYLNLGGRQTARTLMYNF PTRAGQAGGVITSTGKVIGM HVGGNGSHGFAAALKRSYFT QSQGSSGHHHHHH

Data sets:
Data typeCount
13C chemical shifts384
15N chemical shifts146
1H chemical shifts146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PV-3C1

Entities:

Entity 1, PV-3C 193 residues - Formula weight is not available

1   GLYPROGLYPHEASPTYRALAVALALAMET
2   ALALYSARGASNILEVALTHRALATHRTHR
3   SERLYSGLYGLUPHETHRMETLEUGLYVAL
4   HISASPASNVALALAILELEUPROTHRHIS
5   ALASERPROGLYGLUSERILEVALILEASP
6   GLYLYSGLUVALGLUILELEUASPALALYS
7   ALALEUGLUASPGLNALAGLYTHRASNLEU
8   GLUILETHRILEILETHRLEULYSARGASN
9   GLULYSPHEARGASPILEARGPROHISILE
10   PROTHRGLNILETHRGLUTHRASNASPGLY
11   VALLEUILEVALASNTHRSERLYSTYRPRO
12   ASNMETTYRVALPROVALGLYALAVALTHR
13   GLUGLNGLYTYRLEUASNLEUGLYGLYARG
14   GLNTHRALAARGTHRLEUMETTYRASNPHE
15   PROTHRARGALAGLYGLNALAGLYGLYVAL
16   ILETHRSERTHRGLYLYSVALILEGLYMET
17   HISVALGLYGLYASNGLYSERHISGLYPHE
18   ALAALAALALEULYSARGSERTYRPHETHR
19   GLNSERGLNGLYSERSERGLYHISHISHIS
20   HISHISHIS

Samples:

sample_1: 3C, [U-100% 13C; U-100% 15N; 80% 2H], 0.5 mM; HEPES 10 mM; sodium chloride 100 mM; D2O 10%; TSP 1%

sample_2: 3C, [U-100% 15N], 0.5 mM; HEPES 10 mM; sodium chloride 100 mM; D2O 10%; TSP 1%

sample_conditions_1: pH: 8.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker DMX 800 MHz

Related Database Links:

PDB
EMBL CAA24461 CAA24465 CAA25246 CAA25247 CAA25444
GB AAA46914 AAL75969 AAL75970 AAM09803 AAM09805
REF NP_041277 NP_740476 YP_007353734
SP P03300 P03301 P03302