BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15221

Title: Solution structure of Tc32 toxin from the scorpion Tityus cambridgei

Authors: Stehling, Eliana; Sforca, Mauricio; Zanchin, Nilson; Pertinhez, Thelma; Spisni, Alberto

Citation: Stehling, Eliana; Sforca, Mauricio; Zanchin, Nilson; Belluzzi, Ottorino; Pertinhez, Thelma; Spisni, Alberto. "Solution structure of Tc32 toxin from the scorpion Tityus cambridgei"  . ., .-..

Assembly members:
Tc32, polymer, 37 residues, Formula weight is not available

Natural source:   Common Name: Tityus cambridgei   Taxonomy ID: 184226   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Tityus cambridgei

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Tc32: GSTGPQTTCQAAMCEAGCKG LGKSMESCQGDTCKCKA

Data sets:
Data typeCount
1H chemical shifts197

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tc321

Entities:

Entity 1, tc32 37 residues - Formula weight is not available

1   GLYSERTHRGLYPROGLNTHRTHRCYSGLN
2   ALAALAMETCYSGLUALAGLYCYSLYSGLY
3   LEUGLYLYSSERMETGLUSERCYSGLNGLY
4   ASPTHRCYSLYSCYSLYSALA

Samples:

sample_1: Toxin 0.6 mM

sample_conditions_1: pH: 4; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

DYANA, Guntert, Braun and Wuthrich - structure solution

DISCOVER, Accelrys Software Inc. - minimization energy

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
SP P60211