BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15214

Title: MxiHCdelta5 backbone assignment   PubMed: 17827155

Authors: Zhang, Lingling

Citation: Zhang, Lingling; Wang, Yu; Olive, Andrew; Smith, Nathan; Picking, William; De Guzman, Roberto; Picking, Wendy. "Identification of the MxiH needle protein residues responsible for anchoring invasion plasmid antigen D to the type III secretion needle tip"  J. Biol. Chem. 282, 32144-32151 (2007).

Assembly members:
MxiH, polymer, 86 residues, Formula weight is not available

Natural source:   Common Name: Shigella flexneri   Taxonomy ID: 623   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Shigella flexneri

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MxiH: MSVTVPNDDWTLSSLSETFD DGTQTLQGELTLALDKLAKN PSNPQLLAEYQSKLSEYTLY RNAQSNTVKVIKDVDAAILE HHHHHH

Data sets:
Data typeCount
13C chemical shifts50
15N chemical shifts63
1H chemical shifts63

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MxiH1

Entities:

Entity 1, MxiH 86 residues - Formula weight is not available

1   METSERVALTHRVALPROASNASPASPTRP
2   THRLEUSERSERLEUSERGLUTHRPHEASP
3   ASPGLYTHRGLNTHRLEUGLNGLYGLULEU
4   THRLEUALALEUASPLYSLEUALALYSASN
5   PROSERASNPROGLNLEULEUALAGLUTYR
6   GLNSERLYSLEUSERGLUTYRTHRLEUTYR
7   ARGASNALAGLNSERASNTHRVALLYSVAL
8   ILELYSASPVALASPALAALAILELEUGLU
9   HISHISHISHISHISHIS

Samples:

sample_1: MxiH, [U-13C; U-99% 15N], 0.7 mM; NaCl 10 mM; Na Phosphate 10 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB EGW99644
REF WP_001381656 WP_052993805