BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15213

Title: Assignments of {lambda}-IntCB bound to a DNA half-site   PubMed: 18001133

Authors: Kamadurai, Hari; Foster, Mark

Citation: Kamadurai, Hari; Foster, Mark. "DNA recognition via mutual-induced fit by the core-binding domain of bacteriophage lambda integrase."  Biochemistry 46, 13939-13947 (2007).

Assembly members:
Protein, polymer, 86 residues, Formula weight is not available
DNA, polymer, 15 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Protein: TLHSWLDRYEKIASRGIKQK TINYMSKIAIRRGLDATTKE IAANGYIDEGKAASKLIRST LSDAFRAEGHITTHVAATRA AKSEVR
DNA: GCTCAAGGGAGTACG

Data sets:
Data typeCount
13C chemical shifts220
15N chemical shifts86
1H chemical shifts86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1IntCB1
2DNA2

Entities:

Entity 1, IntCB 86 residues - Formula weight is not available

1   THRLEUHISSERTRPLEUASPARGTYRGLU
2   LYSILEALASERARGGLYILELYSGLNLYS
3   THRILEASNTYRMETSERLYSILEALAILE
4   ARGARGGLYLEUASPALATHRTHRLYSGLU
5   ILEALAALAASNGLYTYRILEASPGLUGLY
6   LYSALAALASERLYSLEUILEARGSERTHR
7   LEUSERASPALAPHEARGALAGLUGLYHIS
8   ILETHRTHRHISVALALAALATHRARGALA
9   ALALYSSERGLUVALARG

Entity 2, DNA 15 residues - Formula weight is not available

1   DGDCDTDCDADADGDGDGDA
2   DGDTDADCDG

Samples:

sample_1: Protein, [U-99% 13C; U-99% 15N], 1 mM; DNA 1 mM; TSP 0.02%; sodium phosphate 25 mM; sodium azide 0.02%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, processing

NMR spectrometers:

  • Bruker DRX 600 MHz

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