BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15208

Title: Embryonic Neural Inducing Factor Churchill is not a DNA-Binding Zinc Finger Protein: Solution Structure Reveals a Solvent-Exposed Beta-Sheet and Zinc Binuclear Cluster   PubMed: 17610897

Authors: Lee, Brian; Buck-Koehntop, Bethany; Martinez-Yamout, Maria; Gottesfeld, Joel; Dyson, H. Jane; Wright, Peter

Citation: Lee, Brian; Buck-Koehntop, Bethany; Martinez-Yamout, Maria; Dyson, H. Jane; Wright, Peter. "Embryonic Neural Inducing Factor Churchill is not a DNA-Binding Zinc Finger Protein: Solution Structure Reveals a Solvent-Exposed Beta-Sheet and Zinc Binuclear Cluster"  J. Mol. Biol. 371, 1274-1289 (2007).

Assembly members:
Churchill, polymer, 106 residues, 12166.857 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Churchill: CGDCVEKEYPNRGNTCLENG SFLLNFTGCAVCSKRDFMLI TNKSLKEEDGEEIVTYDHLC KNCHHVIARHEYTFSIMDEF QEYTMLCLLCGKAEDTISIL PDDPRQ

Data sets:
Data typeCount
13C chemical shifts454
15N chemical shifts113
1H chemical shifts734

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2zinc ion, 12
3zinc ion, 22
4zinc ion, 32

Entities:

Entity 1, protein 106 residues - 12166.857 Da.

1   CYSGLYASPCYSVALGLULYSGLUTYRPRO
2   ASNARGGLYASNTHRCYSLEUGLUASNGLY
3   SERPHELEULEUASNPHETHRGLYCYSALA
4   VALCYSSERLYSARGASPPHEMETLEUILE
5   THRASNLYSSERLEULYSGLUGLUASPGLY
6   GLUGLUILEVALTHRTYRASPHISLEUCYS
7   LYSASNCYSHISHISVALILEALAARGHIS
8   GLUTYRTHRPHESERILEMETASPGLUPHE
9   GLNGLUTYRTHRMETLEUCYSLEULEUCYS
10   GLYLYSALAGLUASPTHRILESERILELEU
11   PROASPASPPROARGGLN

Entity 2, zinc ion, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Churchill, [U-13C; U-15N], 1 mM; Tris 25 mM; NaCl 100 mM; DTT 2 mM

sample_conditions_1: ionic strength: 40 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D (HCA)CO(CA)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D CBCGCDsample_1isotropicsample_conditions_1
3D CBCGCEsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
3D HACAHB-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Koll - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - peak picking, refinement

NMRView, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - chemical shift calculation

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 800 MHz
  • Bruker DMX 900 MHz
  • Bruker DRX 750 MHz

Related Database Links:

PDB
DBJ BAG51943 BAG59416 BAH12268
EMBL CAH92555
GB AAH20550 AAI68770 AIC61091 EAW80884 EAW80885
REF NP_001100211 NP_001126496 NP_001189488 NP_001190992 NP_660148
SP Q5R6Q5 Q8WUH1